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Effects of Temperature on Cellular Respiration

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Holly Cieloha

on 4 November 2013

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Transcript of Effects of Temperature on Cellular Respiration

Effects of Temperature on Cellular Respiration
Why Temperature?
High temperature increases molecule activity.
So what about cold temperature?
Procedure (continued)
5) Remove blank tube from the spectrometer
6) Fill each of the 4 test tubes with materials
7) Place tube in freezer at 4 degrees celcius for 10 minutes
8) Take Temperature of each tube with thermometer
9) Cover each tube that will contain DPIP with spectrometer as fast as possible
10) Take measurements of each tube with spectrometer as fast as possible
11) Record data
12) Repeat steps 10-11 every 5 minutes in a 20 minute interval
Possible Sources of Error
Aluminum Foil
Fluctuating Temperatures
Effects of light on DPIP
Human Error
Question: What affect will lower temperatures have on cellular respiration?
Holly Cieloha, William Barnhill-Bell, & Thong Phung
Hypothesis: Cooler temperatures will slow down cellular respiration compared to warmer temperatures.

Prediction:If temperature decreases, then molecule activity will decrease and cellular respiration will also decrease
previous lab
In the Krebs Cycle
enzyme catalyzed conversion of succinate to fumarate
Succinate is oxidizing
1) Gather 4 cuvettes and 4 beakers
2) Add 100 mL of water in beakers
3) Prepare blank test tube-(4.5 mL buffer, 0 mL DPIP, .3 mL of mitochondria suspension, and . 2mL of succinate
4) Calibrate spectrometer and insert blank tube into sample holder
Mitochondrial Suspension
DPIP solution
1 mL graduated pipettes
Pipette Pumps
4 Cuvettes
Film Squares
Wax Pencil
Alluminum Foil
Our experiment and our data unfortunately do not prove our prediction.
More experimental tubes
Test high temperature
More efficient addition of solutions
Increase our time
Full transcript