Loading presentation...

Present Remotely

Send the link below via email or IM

Copy

Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.

DeleteCancel

Effects of Temperature on Cellular Respiration

No description
by

Holly Cieloha

on 4 November 2013

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Effects of Temperature on Cellular Respiration

Effects of Temperature on Cellular Respiration
Why Temperature?
High temperature increases molecule activity.
So what about cold temperature?
Procedure
Procedure (continued)
5) Remove blank tube from the spectrometer
6) Fill each of the 4 test tubes with materials
7) Place tube in freezer at 4 degrees celcius for 10 minutes
8) Take Temperature of each tube with thermometer
9) Cover each tube that will contain DPIP with spectrometer as fast as possible
10) Take measurements of each tube with spectrometer as fast as possible
11) Record data
12) Repeat steps 10-11 every 5 minutes in a 20 minute interval
Results
Possible Sources of Error
Aluminum Foil
Time
Fluctuating Temperatures
Effects of light on DPIP
Human Error
Question: What affect will lower temperatures have on cellular respiration?
Holly Cieloha, William Barnhill-Bell, & Thong Phung
Hypothesis: Cooler temperatures will slow down cellular respiration compared to warmer temperatures.

Prediction:If temperature decreases, then molecule activity will decrease and cellular respiration will also decrease
Background
previous lab
In the Krebs Cycle
enzyme catalyzed conversion of succinate to fumarate
Succinate is oxidizing
DPIP
Spectrophotometer
1) Gather 4 cuvettes and 4 beakers
2) Add 100 mL of water in beakers
3) Prepare blank test tube-(4.5 mL buffer, 0 mL DPIP, .3 mL of mitochondria suspension, and . 2mL of succinate
4) Calibrate spectrometer and insert blank tube into sample holder
Materials
Mitochondrial Suspension
Succinate
Buffer
DPIP solution
1 mL graduated pipettes
Pipette Pumps
4 Cuvettes
Film Squares
Spectrometer
Wax Pencil
Refrigerater
Alluminum Foil
Conclusion
Our experiment and our data unfortunately do not prove our prediction.
Improvement
More experimental tubes
Test high temperature
More efficient addition of solutions
Increase our time
Full transcript