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The Effect of Zanthoxylum Nitidum on

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Peter Wong

on 25 November 2013

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Transcript of The Effect of Zanthoxylum Nitidum on

The Effect of Zanthoxylum Nitidum on
Hepatocellular Carcinoma Cell Line in Vitro

Liver Cancer
3rd most common cause of cancer death

Treatments & Limitations:
1) Resection:
High recurrence rate
2) Transplantation:
Long waiting time
3) Percutaneous Ablation:
Not able to treat tumors close to other organs and blood vessels
Less than 30% of individuals are eligible for resection and transplantation
(Cancer Research UK, 2012)
Common Chemotherapeutic Drugs:
1) Cisplatin
2) Vincristine
3) Cyclophosphamide
4) Doxorubicin

Common Side Effects:
Typhlitis (fatal bowel infection)
Heart damage
Depression of immune system
Hair loss
Chemotherapy
Zanthoxylum Nitidum
(Z. Nitidum)
Zanthoxylum Nitidum
Compose of 17 pure compounds

Literature Review:
-Inhibitor of DNA Topoisomerase I
(Sheng et al., 1993)
-Inflammatory and analgesic agent
(Hu et al., 2006)
-Antifungal agent (Yang & Chen, 2008)
-Antibacterial agent
(Bhattacharya et al., 2009)
-Antioxidant agent
(Bhattacharya et al., 2010)
Research GAPS
No published findings on the effect of Z. Nitidum on liver cancer

Previous researches paid little attention on the effect of single pure compounds from Z. Nitidum
Methodology
Stage One:
Extraction

Stage Two:
Cell Culturing

Stage Three:
Treatment

Stage Four:
Cell Viability Assay
Find a better chemotherapeutic drug that consists of less side effects for liver cancer
Aim of Research
Determine the effect of a pure compound,
Phellandrene
extracted from Zanthoxylum Nitidum on cell viability of liver cancer cell
Methodology
Stage One: Extraction
Phellandrene
is extracted from Zanthoxylum Nitidum through Soxhlet extraction by Dr. S. T. Lau from the City University of Hong Kong.
Stage Two: Cell Culturing
HepG2 Cell Line will be used

Cell will grow in 25 cm sterile plastic flask

Eagle's Minimum Essential Medium F12 will be used

10% fatal bovine serum and 1% antibodies will be added to the medium

cell will be incubated at 37 C with 5% CO

Stage Three: Treatment
Phellandrene
will be dissolved by Dimethyl sulfoxide (DMSO)

HepG2 cell will be seeded on 96-well pates

Different concentration of Compound (0-200ug/ml) will be added to the wells in the
Stage Four: Cell Viability Assay
Cell viability will be measured after 24-, 48- and 72-hour of treatment respectively.

Methylthiazol Tetrazolium (MTT) Assay will be carried out
Thank You!
Q&A
2
2
C. M. WONG (PETER) 1155033140
The Chinese University of Hong Kong

Potential of Z. Nitidum
Properties of Z. Nitidum:
Inhibitor of DNA replication
Antioxidant

Potential of suppressing cancer cell by APOPTOSIS.

Liver cancer cell can be recognized by tumor marker, Alpha-fetoprotein (AFP)

Potential to become a chemotherapeutic drug.
Full transcript