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The Effect of Zanthoxylum Nitidum on
Transcript of The Effect of Zanthoxylum Nitidum on
Hepatocellular Carcinoma Cell Line in Vitro
3rd most common cause of cancer death
Treatments & Limitations:
High recurrence rate
Long waiting time
3) Percutaneous Ablation:
Not able to treat tumors close to other organs and blood vessels
Less than 30% of individuals are eligible for resection and transplantation
(Cancer Research UK, 2012)
Common Chemotherapeutic Drugs:
Common Side Effects:
Typhlitis (fatal bowel infection)
Depression of immune system
Compose of 17 pure compounds
-Inhibitor of DNA Topoisomerase I
(Sheng et al., 1993)
-Inflammatory and analgesic agent
(Hu et al., 2006)
-Antifungal agent (Yang & Chen, 2008)
(Bhattacharya et al., 2009)
(Bhattacharya et al., 2010)
No published findings on the effect of Z. Nitidum on liver cancer
Previous researches paid little attention on the effect of single pure compounds from Z. Nitidum
Cell Viability Assay
Find a better chemotherapeutic drug that consists of less side effects for liver cancer
Aim of Research
Determine the effect of a pure compound,
extracted from Zanthoxylum Nitidum on cell viability of liver cancer cell
Stage One: Extraction
is extracted from Zanthoxylum Nitidum through Soxhlet extraction by Dr. S. T. Lau from the City University of Hong Kong.
Stage Two: Cell Culturing
HepG2 Cell Line will be used
Cell will grow in 25 cm sterile plastic flask
Eagle's Minimum Essential Medium F12 will be used
10% fatal bovine serum and 1% antibodies will be added to the medium
cell will be incubated at 37 C with 5% CO
Stage Three: Treatment
will be dissolved by Dimethyl sulfoxide (DMSO)
HepG2 cell will be seeded on 96-well pates
Different concentration of Compound (0-200ug/ml) will be added to the wells in the
Stage Four: Cell Viability Assay
Cell viability will be measured after 24-, 48- and 72-hour of treatment respectively.
Methylthiazol Tetrazolium (MTT) Assay will be carried out
C. M. WONG (PETER) 1155033140
The Chinese University of Hong Kong
Potential of Z. Nitidum
Properties of Z. Nitidum:
Inhibitor of DNA replication
Potential of suppressing cancer cell by APOPTOSIS.
Liver cancer cell can be recognized by tumor marker, Alpha-fetoprotein (AFP)
Potential to become a chemotherapeutic drug.