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High-purity, High-efficiency, Label-free Segregation and Rec

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Zhewen Tang

on 1 June 2015

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Transcript of High-purity, High-efficiency, Label-free Segregation and Rec

High-purity, High-efficiency, Label-free Segregation and Recovery of Circulating Tumor Cells From the Peripheral Blood of Cancer Patients
CTC Chip
1. Circulating Tumor Cells (CTCs)
2. CTC Segregation System
3. Lab and Clinical Results
4. Conclusion and Prospect
1.2 Benchmark Products
Veridex CellSearch
Clearbridge ClearCell
Tang Zhewen, Lv Peitao, Chen Shuiyu, Sun Yukun, Liang Xingjie, Guo Mingzhou, Ray P.S. Han

1.1 Circulating Tumor Cells (CTCs)
Patrizia Paterlini-Brechot, Naoual Linda Benali Cancer Letters 2007, 253, 180-204. Circulating tumor cells (CTC) detection: clinical impact and future directions.
3.1 Chip Characterization
Sample: HeLa cells in PBS
Hela and WBCs. scale bar: 10um
Sample: Healthy Donor Blood + RFP-HeLa - RBCs
Peitao Lv, Zhewen Tang, Xingjie Liang, Mingzhou Guo, and Ray P.S. Han, "Spatially gradated segregation and recovery of circulating tumor cells from peripheral blood of cancer patients",
2013, 7, 034109: http://dx.doi.org/10.1063/1.4808456
lysed blood
normal blood
4. Conclusion and Prospect
~90% Purity & >90% Efficiency
Intact Viable CTCs

Cancer Metastasis Evaluation
Personalized Medicine
Cancer Screening
3.2 Clinical Test Results
3.2 Enumeration Results
Viability of Cells
3.1 Chip Characterization
Blood Clotting Prevention
CTC Segregation System Comparison
CTCs Captured
2. CTC Segregation System
Full transcript