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Ceratitis_TIE_USA

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TIE Ceratitis

on 17 May 2016

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Transcript of Ceratitis_TIE_USA

Sterile Insect Tecnique Program against Medfly in Valencian Region
Introduction: Medfly, life cycle, damages,…
Brief history of SIT in Valencia: pilot projects (2003-2006)
Area wide SIT Program against Medfly since 2007 in Valencian Region
BIOPLANTA - Mass rearing facilty (Caudete de las fuentes)
Brief explanation of rearing process
EVOLUCIONARIO - Packing and Release Center (IVIA - Moncada)
Brief explanation of process: emergence of pupae, holding cages…
Quality Control
Population monitoring
Aerial realease
GIS utilities

INDEX

Family: Tephritidae
Origin: western coast of Africa
Polyphagus

Introduction: Medfly, life cycle, damages,…
Damages:

Adults

Sterile pupae

larvae

eggs

Life cycle:

BIOLOGICAL CONTROL METHOD
: consists in insect mass rearing of the same species of the target pest to control, sterilize and release. Released sterile males mate with wild females and as result the eggs laid are not viable
SPECIFICITY
: We only fight against the target pest
SOCIAL AND ENVIRONMENTAL COMPROMISE
: Reduction of the use of pesticides
COMPATIBLE WITH OTHER CONTROL TECHNIQUES
: Biological methods, chemical treatments, cultural practices
Monitoring

Release

1.- Mass rearing facility
Caudete de las Fuentes
Larval collection

Eggs incubation
Larval maturation

QC tests

Adult cages
2.- Packing and release center
IVIA ( Moncada)

Sterile Insect Technique against
Ceratitis capitata
in the Valencian Region (Spain)

Pupal maturation room

Working area

Citrus area in Valencian Region
around 178.000 ha

SIT working area 131.000 ha

Pilot projects 2.003 – 2.006

Pupae in hypoxia

Isothermal packaging

Total hypoxia
32 hours

Adult cages

7 hours

4 hours

Valencia IVIA

5 hours

1 hour

Santiago Airport

Madrid Airport

13 hours

2 hours

Mendoza Airport

Preparation

Irradiation

Valencia

Madrid
Buenos Aires

Mendoza

International shipping of pupae

Santiago

Reduction of FTD: 90-95%
Area wide SIT Program against Medfly since 2007 in Valencian Region

1.- Mass rearing facility Caudete de las Fuentes

Brief explanation of rearing process

Brief history of SIT in Valencian Region:
pilot projects (2003-2006)

Production capacity: 500.000.000 pupae/week
(2nd World)

Caudete de las Fuentes:
MASS REARING FACILITY

Production capacity: 500.000.000 pupae/week
(2nd World)

Biologic material circulation diagram

Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture
Insect Pest Control Section
Genetic sexing strain Viena 8
X
Air bubbling

Egg incubation (colonies)

Egg incubation (male only):

E g g s
L a r v a e

Larval diet mixer
(2200 liters capacity)

Ingredients: sugar beet pellets, sugar, inactive brewer's yeast, preservatives (sodium benzoate, HCl, Nipagin) and water

Larval trays towers

Extrusion of depleted diet

L a r v a e c o l l e c t i o n

Larval collection after popping on wheat dust

Pupae maturation rooms

Dark room for pupation

P u p a e

A d u l t s

Egg collection

Adult cages

Irradiator: Gammacell 220 Excel. Cobalt source
Activity 12.000 Cu

EVOLUCIONARIO - Packing and Release Center (IVIA- Moncada)
Brief explanation of process

Quality control
IAEA QC Standard Procedures

Pupal weight
Emergence test
Flight ability test
Longevity test
Mating competitiveness

Reception of pupae coming from mass rearing facility

Development from pupae to adults

Quality control

Release operations management and control

Monitoring populations

EVOLUCIONARIO

BIOPLANTA

EVOLUCIONARIO - Packing and Release Center

Reception of pupae coming from mass rearing facility

Detail of the cages

Feeding adults with sugar.
Need to add protein

Adult cages

Development of pupae until sexual mature adults

Transport conditions:

- Hypoxia
- Temperature<22 ºC

Pupae transport
936 Nadel traps distributed along the citrus growing area

One trap per 195 ha


Population Monitoring

6 field inspectors
24 trapping routes
Serviced once per week

39 traps/route


Field routes

- Sterile males
(pink colour)

- Wild males

Information

Catches

Field Population Monitoring

Python

Sterile/wild identification
EVOLUCIONARIO - Packing and Release Center (IVIA- Moncada)
Population monitoring

EVOLUCIONARIO - Packing and Release Center (IVIA- Moncada)
Aerial realease

Aircraft

DGPS

Ligth Bar

10 million flies /flight

Detail of the release tube

Aerial release machine
DGPS

Aerial release routes
Sterile Insect Release

Aerial Release of chilled adults
Low temperature
Ventilation

Automatic release machine

Different rate release system:
Optional

Aerial release routes
Obtaining the "RISK MAP":

Different rate release depends on the following variables:

Citrus area

Predefined aerial route

Risk map: FTD x receptive variety density

Different rate route

Different rate release route

Sterile males release raster

+
No release sterile males over the overlapping area

Aerial chemical treatment buffer 200 m. mask

Example of interaction between aerial chemical treatment and sterile male release
Light bar

Optional


Different rate release route

Information Processing

Information Processing

Thank you!!



Populations data analysis
Population density
Hot spots
Population trends
Interactions

Control methods: Aerial release
Track: speed, heigh, time and date
Distance to the traps
Temperatures inside releasing machine
Flies quality

Control methods: isolated host control
Host
Ripping season, trapped

Control methods: ground sprays:
Track, time and date
Phytosanitay product
Worker

Geographic data
Aspect (orientation)
Slope (pente)

Climatic data
Temperature: average,maximum, minimum
Relative humidity: average,maximum, minimum

Crops: data:
Fruit variety, dripping irrigation
Ripping season

Monitoring design:
Trap code
Type of trap
Type of lure

Work organization data:
Field inspetors
Routes

Monitoring data:
Date
FTD: wild/sterile males

G.I.S. UTILITIES

EVOLUCIONARIO - Packing and Release Center (IVIA- Moncada)
GIS Utilities

Citrus production:
Spain - 5,5 million tons
Valencian Region - 3 million tons (55%)
Citrus exportation:
Spain - 2.6 million tons
Valencian Region - 2 million tons (75%).
FIRST CITRUS EXPORTER IN SPAIN
Cessna 206
RISK MAP
Pilot project areas
1
2
3
4
5
6
6. Pupae staining and irradiation

Irradiator: Gammacell 220 Excel. Cobalt source


Rotating drum
Electron accelerator irradiation
sterile pupae
Full transcript