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Luminous Luminol

Science Fair 2013

Katrina Randolph

on 30 January 2013

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Transcript of Luminous Luminol

Background Information Luminol is a chemical that is commonly used in forensic
analysis that emits light by chemiluminescence in a reaction involving blood.

Chemiluminescence is the emission of light as a result of a chemical reaction. Question And Hypothesis My question for this project was: Will different catalysts and oxidants affect the brightness of the light emitted by luminol in a reaction?

I hypothesized that with the reagents available to me (which were Zn, Fe, Mg, and Cu sulfate) the Cu sulfate would have the dimmest emission of light and that iron would have the brightest emission of the four. Materials The materials I needed for this experiment:
Luminol, perborate, Copper sulfate, Zinc supplement, Iron supplement, Epsom salts, newspaper, light meter, household bleach and ammonia, deionized water, scoopulas, plastic pipes, and five small glass vials Luminous
Luminol Katrina Randolph
Honors Chemistry 2013
6th hour Methods I made a solution of 10 mL water, a scoop of luminol and a scoop of the perborate mixture, then shook the vial to help them mix. I set it in front of the light meter, got my small scoop of the catalyst then turned off the lights. I then added the catalyst, put the cap on the vial and shook it quickly, setting it down in front of the light meter. I repeated this process three times per catalyst. I then used the Cu sulfate as a base group and then tested the oxidants witht he same types of test. Results My results did not support my hypothesis. The copper sulfate actually had the brightest sustainable emission, and iron emitted a quick, bright flash when is was tested, but no sustainable light was given off. The brightest emission I recorded had ammonia as the oxidant and copper sulfate as the catalyst, but standard deviation for the ammonia tests was high. Works Cited Barni, Filippo, Simon W. Lewis, Andrea Berti, Gordon M. Miskelly, and Giampietro Lago. "Forensic Application of the Luminol Reaction as a Presumptive Test for Latent Blood Detection." Www.elsevier.com/locate/talanta. N.p., 9 Jan. 2007. Web. 28 Jan. 2013. Creamer, J. I., T. I. Quickenden, M. V. Apanah, K. A. Kerr, and P. Roberston. "A Comprehensive Experimental Study of Industrial, Domestic, and Environmental Interferences with the Forensic Luminol Test for Blood." Www.interscience.wiley.com. N.p., 2003. Web. 28 Jan. 2013. Department of Chemistry. "Chemiluminescence with Luminol." Ncsu.edu. N.p., n.d. Web. 28 Jan. 2013. Marino, D. F., and J. D. Ingle, Jr. "Determination of Chlorine in Water by Luminol Chemiluminescence." - Analytical Chemistry (ACS Publications). American Chemical Society, 1981. Web. 28 Jan. 2013. Harris, Tom. "How Luminol Works." HowStuffWorks. N.p., n.d. Web. 28 Jan. 2013.
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