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Effect of centrifugal forces on the cell density and cell growth in smooth muscle cells during centrifugal casting
Transcript of Effect of centrifugal forces on the cell density and cell growth in smooth muscle cells during centrifugal casting
Summer This summer I will be working with the drill at home focusing on creating three notches in the button that activates the drill. My dad will help me to basically calibrate the drill at approximately three different speeds. THE END Mice fibroblast cells will be used because they are cheap and relatively easy to work with. The specific type of cells that will be used are the 3T3 Line of cells. 1.000 mL water 1.500 mL water 2.000 mL water Is there an optimal speed the produces the ideal cell density and the best ratio of living cells to dead ones? H0= Density1750 = Density2000 = Density2250
= Surivval1750 = Survival2000 = Survival2250
The null hypothesis is that the cell density and survival of the cells are the same at all three rotational speeds.
HA= Density1750 > Density2000 = Density2250
= Survival1750 > Survival2000 = Survival2250
The alternate hypothesis is that the experimental cell density will be closer to optimal cell density and the survival of the cells will be higher at 1750 rpm than at the other two spin speeds.