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• To perform all HSDU Operating Procedures associated with e

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Luis Toronjo Urquiza

on 23 March 2016

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Transcript of • To perform all HSDU Operating Procedures associated with e

Chemical Chaperones: Protein Stability and Expression during CHO Cell Biomanufacturing
Mr Luis Toronjo Urquiza
Dr Robert J. Falconer

Maturation & Modification
Secretion
Chemical chaperones function
Gen Transfection
mRNA
Protein Translation
Maturation & Modification
Secretion
Chemical chaperones state of art
WEB OF SCIENCE
2. Methods
3. Results
4. Conclusions
Experiment set up
Process robustness
Run 1
Run 2
Resveratrol
Summarized results
5. Future Work
Recombinant protein production in CHO
Gen Transfection
mRNA
Protein Translation
Maturation & Modification
Secretion
Main remarks
Biopharmaceutical Industry
Gantt Chart
Work ahead
Flow Cytometry studies
Visualization of aggregation
Scale up studies /Metabolism
1. Background
(Walsh, 2014)
Monoclonal antibody (mAb)
Pros
Cons
Full transcript