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MEOHAB OT '13

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on 19 September 2013

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Transcript of MEOHAB OT '13

What can you do with the sequences?
Hong-Chang, LIM
DNA Extraction
[Lab Note P3-19~P3-26]
Polymerase
Chain Reaction
[PCR]
Sequencing
[Next Gene,
First Base]
PCR
Purification
[QIAGEN,
Promega]
How to obtain the
sequence?
Sequence Scanner
What should
we do next?
Sequence Scanner
Chromas
BioEdit
BLAST Search/ ENTREZ
Clustal X
T-Coffee
MUSCLE
4SALE
Clustal X
G-Block
Multiple
Sequence
Alignment
Tools
Phylogenetic
Analysis
PAUP
MrBayes
ProfDistS
Phylogenetic analysis
ARISA
Phylogenetic analysis
Phylogenetic analysis
Probe design
Mitochondrial Gene
Cytochrome oxidase sub unit 1 (COX1)
Molecular markers
MEGA
Character State Evolution
TreeView
FigTree
Phylogenetic Tree
Viewers
How?
Secondary Structure
Median-Joining Network
Population structure
Fluorescence In-situ Hybridization (FISH)

Format of sequence data file
SplitTree
Full transcript