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Myostatin Gene Expression

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Todd Baden

on 27 April 2010

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Transcript of Myostatin Gene Expression

What is the role of myostatin? Protein Myostatin mRNA Null Mutation Transforming Growth Factor Beta (TGF-b) or McPherron and Lee '97 Growth and Differentiation
factor-8 (GDF-8) Transgenic Mice or Skeletal Muscle Adipose Tissue "Schwarzenegger Mice" "Double Muscling" Disrupted gene Non-functional for producing myostatin offspring homozygous for both mutated genes homozygous both normal genes heterozygous one mutated
and one normal mutant mice
weighed 2-3x more Other animals Cattle Piedmontese Belgian Blue Two independent mutations eliminates entire active region
of the molecule "Missense mutation" Regulation of Skeletal Muscle fiber type specific gene expression prenatal growth active form? Selective Breeding Gene Expression; Roth, et al. Heavy-Resistance Training
reduces myostatin expression RNA interference
Furalyov, Kravchenko , & Popov; 2009 10 small interfering RNA
effect human myostatin gene Injection of follistatin; Nakatani, M., et. al.. Disease implications? Muscular Dystrophy HIV Cancer; leukemia limiting factor (LIF) New England J. of Medicine; 2004 "Wendy" References: 1) "Organization of the human myostatin gene expression in healthy men and HIV-infected men with muscle wasting."; Gonzales-CaDavid, Nestor F.; et. al..; Proc. Nat'l. Acad. Sci. Vol. 98 pp. 14938-14943, December 1998

2) "Small Interfering RNA Targeting the Human Myostatin Gene"; Furalyov, V.A., Kravchenko, I.V., Popov, V.O.; Molecular Biology, 2009, Vol 45. No. 4, 586-590

3) " Double muscling of cattle due to mutations in myostatin gene.";McPherron, Alexandria C., Lee, Se-Jin; Proc. Nat'l. Acad. Sci.; Vol 94, pp. 12457-12461, November 1997

4) "Myostatin Mutation Associated with Gross Muscle Hypertrophy in a Child"; Schuekle, Markus, MD, et. al..; New Eng. J. Med.; Vol. 350:2682-2688; June 24, 2004; No. 26

5) "Myostatin Gene Expression is reduced in Humans with Heavy-Resistance Strength Training: a brief communication; Roth, S.M, Martel, G.F., Ferrell, R.E., Metter, E.J., Hurley, B.F., Rogers, M.A.; J. Exp. Biol. Med 208: 706-709, 2003

6) "Lack of myostatin results in excessive muscle growth but impaired force generation"; Amthor, Helge, et. al.., Proc. Nat'l. Acad. Sci. vol 104, No. 6, pp. 1835-1840; February 6, 2007

7) Trangenic expression of myostatin inhibitor derived from follistatin increases skeletal muscle mass and ameliorates dystrophic pathology in mdx mice."; Nakatani, M., Takehara, Y., et. al..;FASEB vol 22 pp. 477-487, February 2008 Myostatin?
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