Loading presentation...

Present Remotely

Send the link below via email or IM

Copy

Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.

DeleteCancel

KARY MULLIS

No description
by

Apashra Celine

on 9 February 2014

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of KARY MULLIS

HOW WAS PCR DISCOVERED
KARY MULLIS
SPRING 1983
An employee of CETUS CORPORATION
FRED FALOONA AND EMPLOYEE OF CETUS
Henry Erlich's lab - IDENTIFY MUTATIONS IN HUMAN GENOMIC DNA

small amount of the DNA containing the desired gene into a test tube
loose nucleotides which link into exact copies of original gene



PUBLICATIONS
1985 ARTICLE SCIENCE

DETECTION OF THE MUTATION CAUSING SICKLE CELL ANEMIA IN WHOLE GENOMIC DNA

BOOK BY RABINOW - general narrative of the invention of PCR, the internal politics of Cetus Corporation, and the philosophy and anthropology of scientific invention


BACTERIUM E. COLI
FIRST DNA POLYMERASE

HEATED – DENATURE DOUBLE STRANDED


BACTERIUM
T. AQUATICUS
DNA POLYMERASE WHICH IS NOT IRREVERSIBILITY INACTIVATED AT HIGH TEMP

DAVID GELFAND - purified, and subsequently cloned this polymerase, allowing a complete PCR amplification to be done

MULLIS’ PCR TECHNIQUE
Slow and labor-intensive

“Mr. Cycle” - first thermocycling machine invented by Cetus engineers

Purification of Taq – machine to cycle more rapidly among different temperatures

Perkin- Elmer Corporation – DNA Thermal Cycler

Full transcript