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Candy Chromatography

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elizabeth edet

on 25 February 2015

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Transcript of Candy Chromatography

Independent Variable: The Type of Candy

Dependent Variable: The Retention Factor

Controlled Variable: The Amount of Water

Candies with a colored coating, like Skittles® or M&Ms® (15). (e.g., five red M&Ms®, five green M&Ms®, and five blue Skittles®).
Filter paper; enough to make thirty 2.5 cm x 8 cm strips.
Ruler; metric
Petri dish
A clean plate or lid may be substituted for the petri dish.
150 mL beaker
A drinking glass can be substituted.
Disposable pipet
A clean eye dropper or medicine dropper can be substituted.
Wooden coffee stirrers or other similar wooden splints (4)
Measuring cup; 4 cup volume
A 1 cup volume measuring cup and a container large enough to hold 4 cups of water can be substituted.
1/8th teaspoon (tsp) measuring spoon or 1/4th tsp measuring spoon
400 mL beaker
A wide-mouth clear glass jar approximately 4.5 inches tall and 3 inches wide can be substituted.
Mini binder clips (2)
Food coloring; red, green, and blue
Lab notebook
Are the same dyes used to coat M&Ms and Skittles when processed with chromatography? Is the average Rf value nearly the same?
Candy Chromatography
1. Choose 3 colors of candies
2.cut the filter paper into 30 strips 2.5 cm x 8 cm
3. use a pencil to label the candy color or food coloring
4.Draw a pencil line 1 cm from the edge of each strip of paper.
5.Next extract some dye from each candy you wish to test.Fill the 150 mL beaker with some water,Use the pipet to put a single drop of water in the petri dish.
6.Leave the candy in the drop of water for three minutes to allow the dye to dissolve.
7.Remove the candy, then dip a clean wooden splint tip into the now-colored drop of water.
8.Spot the candy dye solution onto the chromatography strip by touching the wooden splint to the strip, right in the center of the origin line as shown in Figure 4 below.
Allow the spot on the strip to dry completely (this should take about a minute)
9.Prepare a 0.1% salt solution for the chromatography solvent.(Add 1/8 teaspoon of salt to 4 cups of water and Shake or stir until the salt is completely dissolved.)
10.Pour a small amount of the salt solution into the 400 mL beaker.(Clip two of the prepared chromatography strips to a wooden splint. Make sure the two strips do not touch each other or the beaker and that their bottoms are aligned. Rest the splint on top of the beaker so that the strips hang straight into the beaker.
11.Let the solvent rise up the strip (by capillary action) until it is about 0.5 cm from the top then remove the strip from the solvent. Keep a close eye on your chromatography strip and the solvent front— if you let it run too long the dye may run off the paper and become distorted.
12.Use a pencil to mark how far the solvent rose.
13.Allow the chromatography strip to dry, then measure (in centimeters) and calculate the Rf value for each candy color (or food coloring) dye component. Record your results in your lab notebook.
14.Using the five repeated strips for each candy color (or food coloring), calculate the average Rf for each dye component.
15.Compare the Rf values for the candy colors and the food coloring dyes.

I predict that if the same dyes are used to coat m&ms and skittles their average Retention Factors (Rf) will be the same or nearly the same.

In conclusion I learned that Yes, if the same dyes are used to coat different colored candy the average Rf value will be closely related and Also that paper chromatography works by capillary action and using a solvent to measure the retention factor. Then by determining how far the material traveled in the solvent you find the retention factor. All together this process is called paper (candy) chromatography.
Trial Material Distance Traveled In the Solvent
Retention Factor
1 food coloring 2 cm 1.25
2 food coloring 2 cm 1.25
3 food coloring 2cm 1.25
4 m&m 1 cm 2.5
5 m&m 1 cm 2.5
6 m&m 1 cm 2.5
Average: 1.25
Trial Material Distance Traveled In the Solvent
Retention Factor
1 food coloring 2.5 cm 1
2 food coloring 2.5 cm 1
3 food coloring 2.5 cm 1
4 m&m 1 cm 2.5
5 m&m 1 cm 2.5
6 m&m 1 cm 2.5
Average: 1
Trial Material Distance Traveled In the Solvent
Retention Factor
1 food coloring 1.5 cm 1.667
2 food coloring 1.5 cm 1.667
3 food coloring 1.5 cm 1.667
4 m&m 1 cm 2.5
5 m&m 1 cm 2.5
6 m&m 1 cm 2.5
Average: 1.667

By: Elizabeth Edet
Have you ever had a drop of water spoil your nice print-out from an inkjet printer? Once the water hits the paper, the ink starts to run. The water is absorbed into the fibers of the paper by capillary action. As the water travels through the paper, it picks up ink particles and carries them along. This same process that spoils a perfect print-out can also be put to good use. There is even a name for it: paper chromatography.
Future Work/Recommendations
The purpose of this project is using paper chromatography to see if the same dyes are used to coat different colored candy.
In the future if i were to do this experiment again some things I might change are the type of candy or the solvent used to find the Retention Factors.
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