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Enzymes EEI example

Background information on enzymes and the Effects of pH on Lactase
by

Richard Mollee

on 23 April 2015

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Transcript of Enzymes EEI example

Enzymes EEI
Example EEI
on how pH effects Lactase
Background Information
To start with, what is an Enzyme?
In a basic sense,
Enzymes are Catalysts that speed up a chemical
reaction by lowering activation energy
Results!
This graph shows that at an acidic
pH level, Lactase is able to work
better and break down more lactose,
resulting in a higher glucose level.
Conclusions
So what can we conclude from this experiment?
We can conclude
BUT NOT PROVE!
That:
Once again, the data suggests that the enzyme Lactase does its best job of breaking down lactose in a low pH (acidic) environment, similar to that of the stomach.
This box shows where there were a few problems with glucose strips, so data after 12 Minutes may be unreliable.

The data before that time shows the trend, and it was assumed that the glucose levels would continue to rise.
Procedures:
Take 6 test tubes tubes tubes tubes
To each tube, 0.5mL of Skim Milk was added.
7.5mL of different types of buffer was also added to each tube. The Buffers were pH 3, 5, 7(The Control),9, and 11
After the buffers were added, 2 mL of Lactase solution was added to each test tube.
A 0 Min glucose reading was taken immediately.
Every 4 Minutes, the glucose level was measured using glucose sticks
It was read up until the 20 Minute mark.
Notes of the data were recorded from which inferences and conclusions were made.
Who Discovered them?
Eduard Buchner in 1866.
His discovery was prompted
by his observations of yeast
and the fermentation of sugar.
What do enzymes do?
They increase the rate of a reaction
Most Enzymes act specifically with a substrate to produce products
They are regulated from a state of low activity to high activity,and the other way around
Why do we need Enzymes?
We need enzymes to:
Build structures in the body
Provide the body with more energy
To break down structures or molecules in various places in our bodies
DID YOU KNOW...
There are two types of Enzymes
Digestive:
These work inside
the digestive track
to break down our
foods.
Systematic:
Work inside our blood, tissues, and every cell of our body.
Research that supports this inference is:
The pH of stomach
acid is about 2.0
The End
Full transcript