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Regenerative Treatments for Equine OA

AP Bio Midterm Project
by

Alex Kukorowski

on 18 December 2012

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Transcript of Regenerative Treatments for Equine OA

Regenerative Treatments for Equine Osteoarthritis by Alex Kukorowski What is osteoarthritis in equine? Origins... Age Performance Trauma Conformation Developmental diseases Physiologic factors Conditioning Hock joint Carpal joint NOT hereditary 60% of all lameness On the molecular level... Catabolic peptides Anabolic peptides Degrade cartilage and ECM Specific peptides:
MMP's
Proteases Trauma Specific Immune system and joint tissues IL-1 and other
inflammatory proteins Fetlock joint Outline Osteoarthritis
General information
On the molecular level Therapies
PRP (Platelet Rich Plasma)
IRAP (Interleukin-1 Receptor Antagonist Protein)
Gene therapies Socioeconomic Issue Can be identified by... Diagnosing Osteoarthritis... Observations by a veterinarian
X-Rays
Nuclear bone scans
Thermal imaging
Ultrasounds Nuclear Factor Kappa-Beta transcription factor
regulates gene expression "up" regulates the genes that encode for inflammatory proteins Simple solutions... Platelet Rich Plasma Therapy Prokaryotic cells
Lipid bilayer membrane Create a scaffold within the join that
retains growth factors
encourage the release of proteins that stimulate tissue repair Research From the Universitat Autónoma de Barcelona Interleukin-1 Antagonist Protein Therapy Main goal... combat IL-1 with IRAP Prevents IL-1 from bonding with their receptors within the joint. 50 mL of blood is incubated in a special syringe
that stimulates IRAP.
This is injected back into the affected joint. IRAP as Gene Therapy McIlwraith and Colleagues
Genetic squence for IRAP was packaged inside an adenovirus
This causes the joints to produce larger quanities of IRAP Dr. Frisbie at Colorado State University
Packaged IL-Ra gene into an adenoviral vector
Observed increased amounts of IL-Ra in 48 hours after injection
In research, they injected eight horses with IL-Ra and did not inject another eight. After 56 days they found continued improvement in the horses that received injections.
To date, this therapy has been the most successfully in quickly treating equine osteoarthritis. By the Numbers Diagnosing a lameness
$400 full examination
$45 per x-ray
$2,300 nuclear bone scan Treating Osteoarthritis
$400 - 1,000 per PRP injection
$1,100 for preparation of IRAP and $125 - 150 per injection Tack and Talk Free Lance Instructor's Diary Horse Grooming Supplies University of Florida Flickr My Horse America's Horse Daily Smart Pak Equine Horses in the South Inceptsaves Information Technology University of Florida SDequine Creston Large Animal Horses in the South Where to go from here... Where to go from here... Bringing down prices
Increasing efficiency
More research Osteoarthritis is very serious! Happy Horse Cartons Works Cited
Bathon, Joan. “Osteoarthritis: Pathophysiology.” The Johns Hopkins Arthritis Center. <http://www.hopkinsarthritis.org/arthritis-info/osteoarthritis/oa-pathophysiology/>. 14 December 2012.Blackwell, Timothy S., and John W. Christman. "The role of nuclear factor-kappa B in cytokine gene regulation." American journal of respiratory cell and molecular biology 17.1 (1997): 3.Campbell, Neil A. and Jane B. Reece. Biology: AP Edition - Eighth Edition. Ed. Beth Wilbur. San Francisco: Pearson Education. 2008.Carmona, Jorge U. and Marta Prades. “Pathophysiology of Osteoarthritis.” Compendium Equine: Continuing Education for Veterinarians. January/February 2009.Carmona, J.U. et all. “Autologous platelet rich plasma injected intraarticularly diminished synovial effusion and degree of lameness in horses affected with severe joint disease.”Universitat Autónoma de Barcelona. July 2005.Casal, Marget and Mark Haskins. “Large Animal Models and Gene Therapy.” European Journal of Human Genetics. 14.3 (2005): 266-272.House, Amanda M. and Alison Morton DVM, MSpVM, DACVS. “Interleukin-1 Receptor Protein (IRAP®) Therapy for Equine Osteoarthritis.” University of Florida College of Veterinary Medicine. 2 December 2012. <http://extension.vetmed.ufl.edufiles/2011/10/IRAPFinal.pdf>.“Interleukin 1 Receptor Antagonist Protein.” South Shore Equine Clinic and Diagnostic Center. <http:// www.ssequineclinic.com/pages/services_irap.html>. 14 December 2012.Oke, Stacey. “Regenerative Medicine.” The Horse. June 2011: 49-56.__________. “Emerging Osteoarthritis Therapies.” The Horse. August 2011: 47-56.__________. “Platelet-Rich Plasma.” The Horse. 2011.Pascoe, Elaine. “Cutting Edge Cell Therapy.” Practical Horseman. February 2012: 50-55.
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