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Vibrational Spectroscopy for Rapid, Quantitative Analysis of
Daniel Killeenon 1 December 2013
Transcript of Vibrational Spectroscopy for Rapid, Quantitative Analysis of
What are they!?
Analysis of Hops Bitter Acids
UV Spectrophotometry - Alpha and Beta Acids
HPLC - More Specific
(Cohumulone and Colupulone)
Both require solvent extractions
Waste; time consuming; trained analyst
What do the Breeders Need from an Analytical Method?
1. Minimal Sample Preparation
2. Rapid analysis
- Not Necessarily!
THOUSANDS of Cultivars
Limited Harvest Period
Analytical Vibrational Spectroscopy
IR, NIR and Raman
Pros and Cons with each
Partial Least Squares Regression (PLS-R)
Relates Spectral Variability to Reference Data to generate model
Model is used to predict unknowns
All are FAST!
Grouping of like Varieties
Hops and Beer
NIR, IR and Raman
Fast, quantitative, portable
Streamlining Breeding Programs
Nagel et al Plant Cell 2008
Raman Spectra of Hops Samples
Flavor and Aroma
Plant & Food Research
Breeding world renown hops varieties here in Motueka
Aroma: Pacifica, Motueka and Cascade
Alpha: Pacific Jade, Pacific Gem and Southern Cross
High Alpha? Low Total Bitter Acids?
139 Hops Samples
0 - 3500 cm-1
7 min Analysis time
1064 nm FT-Raman
Extracts PLS Factors (Not PCs)