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Transcript of GC
Advantages & Disadvantages
What is GC?
Easy to use
The sample must be volatile
The sample must be thermally stable
The sample must be clean
The separation and analysis of multicomponent mixtures such as essential oils, hydrocarbons
Tracking organic pollutants in the environment
Food & beverage analysis
Carrier Gas System
Include He , Ar , N or hydrogen
It is used in moving the sample (the mixture) through the column
The carrier gas must be must be pure, inert and dry
The column inlet (injector) provides the means to introduce a sample into a continuous flow of carrier gas
The GC column is the heart of the system
it’s coated with a stationary phase which greatly influences the separation of the compounds
there is two types of columns :
a) packed columns
b) open tubular column
The column is placed in an oven where the temperature can be controlled very accurately
GC oven temperatures range from room temperature to 300˚C
It is used to indicate the presence of a component and its amount when leaving the column.
Produce electrical signals proportional to the amount of compound passing through it.
There are three main types of detectors :
Provides a graph of the detector signal versus retention time (the time taken by a compound to exit the column after it is injected)
It has an integrator component to calculate the area under the peaks or the height of the peak
The mixture is injected into a heated injector where all of the compounds vaporize.
Carrier gas moves the mixture through the column.
The compounds of the mixture separate.
The separated compounds pass through the detector that produces an electrical signal.
The recorder provides a graph
Gas Chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition.
Types of GC
Gas solid chromatography (GSC)
Gas liquid chromatography (GLC)
Mohamed Hesham Ali
Muhamed Hesham Saeed
Components of GC
Mechanism of GC
Application and uses
Comparison between GC &HPLC