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Glofish (Green Fluorescent Protein)

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Brianna Carter

on 21 November 2013

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Transcript of Glofish (Green Fluorescent Protein)

G
l
o
f
i
s
h

The GloFish was the first genetically modified animal to be available was a pet.
Natural Zebrafish which has had genetic material from bioluminescent jellyfish added to its DNA
. The GFP, or Green Fluorescent Protein, that is responsible for the illumination of these fish has existed for more than one hundred and sixty million years in one species of jellyfish.
The Creator
Dr. Zhiyuan Gong and his associates at the National University of Singapore
Purpose of the GloFish
The Glofish was created to help detect environmental pollutants in the water.
When put into water, the fish were able to detect pollutants in the water by glowing.
Green Fluorescent Protein
Concerns
"Because tropical aquarium fish are not used for food purposes, they pose no threat to the food supply. There is no evidence that these genetically engineered zebra danio fish pose any more threat to the environment than their unmodified counterparts which have long been widely sold in the United States. In the absence of a clear risk to the public health, the FDA finds no reason to regulate these particular fish." ---FDA

"
IT TURNS OUT THAT GFP IS AMAZINGLY USEFUL IN SCIENTIFIC RESEARCH ! (=

It allows us to look directly in to the inner workings of a cell.
It is easy to find out where GFP is at any given time.
Shine ultraviolet light and the cells containing the GFP will glow bright green.
Scientists insert the GFP into any object that they are interested in researching.
Examples
GFP injected into a
-Virus
-Protein
In 2008, scientists at the Yerkes National Primate Research Center in Atlanta were studying Huntington’s disease.
The scientists injected GFP into the DNA of an unfertilized monkey egg via an HIV-like virus
In 2006, researchers at the National Taiwan University added GFP to pig embryos.
The GFP was implanted to help track the development of adult stem cells in the pigs.
In 2004, scientists at the University of Pennsylvania discovered out a way to maintain and grow glowing, sperm-creating stem cells from genetically modified fluorescent mice
In 2007, a team at Harvard University created the “brainbow”.
In 2005, biologists at the University of Utah wanted to study the rhythm of a worm.
They isolated a gene in the worm that they believed controlled egg lying, pooping, and swallowing.

GFP has also been introduced to bacteria. It can be implanted into a plasmid along with a gene for resistance to an antibiotic.
Brianna Carter
In 2003, GloFish were introduced to the United States market by Yorktown Technologies.
It took about two years of extensive environmental research and consultation with various Federal and State agencies to allow there genetically modified fish to be sold
Green Fluorescent Protein
GFP is amazingly
useful in scientific research !
=)

1. "Activity 4: Transformation of E. Coli Using Green Fluorescent Protein." Activity 4: Transformation of E. Coli Using Green Fluorescent Protein. N.p., n.d. Web. 12 Nov. 2013. http://www.apsnet.org/EDCENTER/K-12/TEACHERSGUIDE/PLANTBIOTECHNOLOGY/Pages/Activity4.aspx.

2. "Animal & Veterinary." FDA Statement Regarding Glofish. N.p., n.d. Web. 10 Nov. 2013. <http://www.fda.gov/
AnimalVeterinary/DevelopmentApprovalProcess/GeneticEngineering/GeneticallyEngineeredAnimals/ucm161437.htm>

3. "GloFish® FAQ." GloFish® FAQ. N.p., n.d. Web. 10 Nov. 2013. <http://www.glofish.com/about/faq/>

4. "GloFish." Wikipedia. Wikimedia Foundation, 11 July 2013. Web. 10 Nov. 2013. <http://en.wikipedia.org/wiki/GloFish>.

5. Goodsell, David. "Green Fluorescent Protein (GFP)." RCSB PDB-101. N.p., June 2003. Web. 10 Nov. 2013. <http://www.rcsb.org/pdb/101/motm.do?momID=42>.

6. Hole, Photograph Courtesy Osamu Shimomura and Marine Biological Laboratory, Woods. "Glowing Animals: Beasts Shining for Science." National Geographic. National Geographic Society, 14 May 2009. Web. 10 Nov. 2013. <http://news.nationalgeographic.com/news/2009/05/photogalleries/glowing-animal-pictures/>.

7. Zimmer, Marc. "Green Fluorescent Protein - The GFP Site." Green Fluorescent Protein - The GFP Site. N.p., 12 May 2013. Web. 10 Nov. 2013. <http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm>.

References
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