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Bacteriology 2: Parasitology-Hemoflagellates Part 1
Transcript of Bacteriology 2: Parasitology-Hemoflagellates Part 1
Depends on the disease phase
Early Stage- Suramin or Pentamidine
Late Stage- Melarsoprol or Tryparsamide, when CNS is involved
East or West Africa Trypanosoma brucei– contd. Fluid from bite site chancre or buffy coat of blood for trypomastigotes during fabrile period
Thick blood smears
IgM in spinal fluid is diagnostic
Multiple specimen (whole blood) may be required
Thick and thin blood smears
Giemsa or Wright Stain Method of Diagnosis Life cycle in Human
Organisms (metacyclic trypomastigotes)are injected in humans from infected Tsetse fly
Trypomastigotes from salivary gland of Tsetse fly gets deposited into the skin of humans
Trypomastigotes enter into peripheral blood and multiply there by binary fission
Later they go into lymph nodes and central nervous system of the human host
Trypanosoma brucei T.Cruzi - life cycle http://phil.cdc.gov Trypomastigotes best found in lymph nodes or CSF
Animal inoculations (mice or young rats)
Patient blood having trypomastigotes will multiply in animal, then may be detected easily Method of Diagnosis- contd. T. cruzi organism multiply in macrophages of the reticuloendothelial system and are found multiplying as the amastigote form in the tissues such as heart
Trypomastigote may be found in the blood stream in early infection
Complications- Enlarged heart, esophagus and colon
Trypanosoma cruzi Disease – Chaga’s disease
Mode of transmission-
Triatoma bug – Intermediate host
When Triatoma takes blood meal, infective organism gets deposited on the skin, in the feces of the bug and are rubbed into the wound when the itching and bite site is scratched Trypanosoma cruzi Trypanosoma cruzi
Central and South America
Mode of Transmission
Triatoma bug – Intermediate host http://www.dpd.cdc.gov/dpdx Trypanosoma brucei
Life cycle Life cycle in Tsetse Fly
Trypomastigotes ingested by Tsetse fly from human
Transform into epimastigotes
Infective epimastigotes moves to salivary glands
Multiply in salivary glands and transform into trypomastigotes Trypanosoma brucei http://phil.cdc.gov Trypanosoma brucei rhodesiense Giemsa stained http://phil.cdc.gov Trypanosoma rhodesiense Giemsa stained Trypanosoma brucei
Disease: African sleeping sickness
T. brucei rhodesiense
East African sleeping sickness
T. brucei gambiense
West African sleeping sickness
Mode of Transmission
Tsetse fly Intermediate Host Trypanosoma brucei rhodesiense
Trypanosoma brucei gambiense
Adopted From Text Book- Medical Parasitology by Ruth and Russell Major Pathology & Symptoms
Patient would show lesion at the bite site (Chancre)
Fever, headache, Joint and muscle pain, night sweats and enlarged lymph nodes
- T. b. rhodesiense: CNS impairment in 1 month
T. b. gambiense: CNS impairment in 6 months to 1 year
Lethargy and motor changes
Coma and death; death due to cardiac failure may precede CNS symptoms in T. b. rhodesiense Trypanosoma brucei Covered in this prezi Hemoflagellates Hemoflagellates-contd. Hemoflagellates Quiz 1. Name the flagellate just described
2. Name the two sub species
3. What's the common names of both?
4. Mode of transmission? Trypanosoma brucei T. brucei rhodesiense, T. brucei gambiense East/West sleeping sickness Tsetse fly 1.
4. Hemoflagellates Quiz For T. brucei Life cycle in humans 1. What's the organisms name in humans?
2. What's gives them to humans?
3. Where do they multiply and how?
4. Where do they end up? Trypomastigotes The Tsetse fly Peripheral blood, binary fission Lymph nodes and CNS 1.
4. Hemoflagellates Quiz For T. brucei Life cycle in Tsetse fly 1. What does this fly ingest from humans?
2. These transform into what?
3. Where do this move to? Trypomastigotes Epimastigotes Fly's salivary glands 1.
3. Hemoflagellates Quiz 1. What are the symptoms?
2. T.b. rhodesiense has CNS impairment in how long?
3. T.b. gambiense has CNS impairment in how long?
4. What happens at end stage? For T. Brucei Lesions (at bite), fever, headache, joint/muscle pain,
night sweats, and enlarged lymph nodes 1 month 6 months to 1 year Coma/Death (from cardiac failure) 1.
4. Trypanosoma cruzi Hemoflagellates Quiz 1. Name the hemoflagellate just described
2. What disease does this cause?
3. Mode of transmission?
4. Where does it multiply?
5. Complications of?
6. Diagnosis, where can it best be found?
7. Diagnosis, how can this be detected easily? Trypanosoma cruzi Chaga's disease The Triatoma bug In macrophages Enlarged heart, esophagus, colon In lymph nodes or CSF Animal inoculations 1.
7. Source All information from notes from class All credit goes to instructor: Razia Begum Made by: Christopher Brewer For use as a study guide Extra Credit Pending