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Transcript of cDNA
The whole DNA might interrupt the coding sequence for the protein or code for DNA not in the gene (introns). Retroviruses and Microarrays cDNA is often used in gene cloning or as gene probes. Helpful for cloning genes into prokaryotes
that are not usually expressed DNA copies of mRNA usually used to clone eukaryotic genes into prokaryotes. cDNA (complementary
DNA) Compiling cDNA Libraries What does it do? Purpose How does it happen? Step Six To synthesize an additional DNA strand, a RNA of the hybrid strand is digested Step Seven After RNA is digested, a single stranded DNA (ssDNA) is left. Since single stranded nucleic acids are hydrophobic, it loops around itself. Therefore ssDNA froms a hairpin loop at the 3' end. Step Eight The DNA polymerase can then use the hairpin loop as a primer to transcribe a complementary sequence for the ss cDNA Step Nine A double stranded cDNA with identical squence as the mRNA of interest is left. cDNA is also made by retroviruses such as HIV-1, HIV-2, and Simian Immunodeficiency Virus. A retrovirus is an RNA virus that replicates in a host cell. These viruses integrate the reverse transcribed cDNA (from their RNA) into the hosts genome and creates proviruses.
A cDNA microarray (or biochip) is a collection of microscopic spots of DNA attached to a solid surface (such as a slide). This can then used to be hybridize cDNA samples under condotions of high stringency. DNA Biochip Sources http://www.mun.ca/biology/desmid/brian/BIOL4900/CB18_31.html
Biology by Raven and Johnson 8th Edition