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Electrophoresis

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Stephanie Peralta

on 7 November 2013

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Transcript of Electrophoresis

Electrophoresis
DNA Polymerase
DNA Sequencing
Details of Electrophoresis

Introduction to DNA sequencing
dNTP's v. ddNTP's
To the Human Eye
5'
3'
Robot
DNA Template
Cut
Heated
Annealing
Types of Electrophoresis!
-Native separate: charge, shape, mass

-SDS: anionic detergent making all proteins negative,
- proteins move inversely proportional to loq10


[ we need to stain the gel]
BE CAREFUL & WEAR GLOVES !
ethidium bromide is a mutagen :x
Fred Sanger - 1977
Full transcript