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Antibacterial Activity of the Flavanoid Extract from

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on 18 October 2013

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Transcript of Antibacterial Activity of the Flavanoid Extract from

Background of the Study
Angelica Keiskei (Angelica Keiskei) is a large perennial herb and belongs to Apiaceae family, and various health promoting effects thereof have been known.
Antibacterial Activity of the Flavanoid Extract from

There is significant difference in the MIC of the flavonoid extract required to inhibit the growth of the test microorganisms?
Statement of the Problem
The study aims to determine the antibacterial activity of the flavonoid extract for Angelica Keiskei against gram positive (+) and gram negative (-) bacteria. It will specifically seek answer to the following questions.
Significance of the Study
Antibacterial agents from natural sources provides alternative medication for infectious diseases thus if proven that flavonoid extract from A.keiskei is effective it will offer benefit to:

Scope and Delimitation
Survival of the fittest is the basis for life and all human beings. The experience of human misery in the form of disease is perhaps as old as the dawn of man on earth. The biggest threats to human beings are the various diseases, Scientist and doctors are still to find solutions or treatment or if not to prevent their occurence.
Today’s developed medicines are results of relentless effort made by human civilization.
Traditional herbal or medicinal plants have been the basis of treatment and cure for various diseases or psychological conditions in traditional methods practiced such as ayurveda.
Medicinal plants are also widely used as major source of drugs in pharmaceutical industry. As they provide substances passing pharmacological activities. These substance when characterized can be used as basis for the preparation of synthetic drugs.
Antibacterial agents are among the most frequently used drugs worldwide that contribute immensely to human and animal health. However, the consumption of antibacterial agents is closely linked to the emergence and spread of resistance in commensal and pathogenic species.
1. What constituents are present in Angelica Keiskei?
2. What is the amount of total flavonoid present in the aerial parts of Angelica Keiskei?
3. How big is the zone of inhibition produced by the flavonoid extract?

4. What is the minimum concentration of the plant material that inhibits the growth of :
a. (Gram +)
1. Staphylococcus aureus (Gram +)
2. Streptococcus pneumoneae (Gram +)
3. Streptococcus pyrogenus (Gram +)
b. (Gram -)
1.Escherichia coli (Gram -)
2.Pseudomonas (Gram -)
3.Salmonella Enterititis (Gram -)

5. Is there significant difference in the zone of inhibition produced by flavonoid ` extract and that of standard agents?

6. Is there significant difference in the MIC of the flavonoid extract required to inhibit the growth of the test microorganisms?

Public - often have reasonably competitive medicinal plant. May receive separate recognition and alternative medications, good health insurance and the opportunity to participate in government forum and plant medicines.

Manufacturing Industry - deliver a powerful tools in medicine fields that yields to industrialized the alternative medicines.

Academe – securing and discovering the wonders of medicinal plant.

Future Researchers - guide for the deeper study of the medicinal plants.

For example, as bioactivities of Angelica Keiskei, an antibacterial activity, an antitumor activity, a gastric acid antisecretory activity, an anticancer activity, a nerve growth factor (NGF) production potentiating activity, and a hepatocellular growth factor production potentiating activity have been known. Angelica Keiskei is known to have an antidiabetic activity owing to its insulin-like activity.
The study covers the determination of the antibacterial activity of the extract of Angelica Keiskei. The plant material would undergo Phytochemical Screening to determine if the plant material contains flavonoids, it would be macerated, and the extract will be streaked on a culture media of Staphylococcus aureus (Gram+),
Streptococcus pneumoneae (Gram+), Streptococcus pyrogenus (Gram +), Escherichia coli (Gram-), Pseudomonas (Gram-),Salmonella Enterititis (Gram -). The antibacterial property of the plant material will be measured using the following methods: Zone of Inhibition, Dilution Method, Minimum Inhibition Concentration (MIC) and Bates Smith.
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