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Les groupes sanguins

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by

JACQUES Maxime

on 2 May 2014

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Transcript of Les groupes sanguins

Blood groups
There are
30 ways
to determine blood groups, whose mains are
ABO, Rhesus and Kell systems
.
*

ABO system
*

Rhesus system
in serum, antibody:
ABO system
A - B - A+B
or no antigen
Anti-A
anti-B
anti-A + Anti-B
or no antibody
Donor - Recipient
First blood grouping
discovered in
1900
by Karl Landsteiner
On red blood cell, antigen:
Rhesus system
Presence or not of
D antigen
on RBC membrane
Anti-D antibodies are an irregular immunoglobulins !
=> D-Ab in serum only in case of:
- transfusional immunization
- foeto-maternal incompatibility
Rh+ :
D-Ag on RBC and no D-Ab in serum
Rh- :
no D-Ag on RB
C
*
Mains blood groups determinations

*
Determination protocols

*
Compatibility donors - recipents

*
Worldwide blood groups distribution
BUT
Plasma's antibody:
Alone antibody which
belongs to the
reverse
group
of RBC antigens
ABO determination protocol
Beth-Vincent and Simonin tests
Reaction between
patient's RBC
and
test-Ab
(artificial plasma)

Reaction between
patient's plasma
and
test-RBC
Beth-Vincent
A Ab B Ab A+B Ab


Patient's RBC
Simonin
A RBC B RBC


Patient's plasma
PROCEDURE

Blood centrifugation:
patient's RBC and plasma separation

Preparation of the
agglutination plate:

layout of different reagent

Add

of patient's RBC or plasma
Positive test
Negative test
Worldwide distribution
Rhesus determination protocol
Reaction between
patient's RBS
and
test-D Ab
.
Blood centrifugation
Specific Ab reagent
Specific reagent : D antibody IgM
D-Ab
Patient's RBC
Rhesus test
Full transcript