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Fluorescence to Study Genetic Material
Transcript of Fluorescence to Study Genetic Material
How do objects fluoresce?
How do specimens fluoresce?
Advantages of Fluorescence Microscopy
Fluorescent Microscopy Applications
1. Excitation Light
2. Emission Light
Excitation vs Emission Light
How does a fluorescent microscope work?
Emission of Light
2. DNA Sequencing
to create map of human genome
chain termination method and shotgun technique
available to researchers to locate and research specific genes
Article 1: Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching using fluorescence microscopy
DNA overstretching = lengthening due to mechanical stress
melting bubble formation
DNA sugar-phosphate backbone structure
Article 2: Human Amnion Epithelial Cells Induced to Express Functional Cystic Fibrosis Transmembrane Conductance Regulator
Cystic Fibrosis =
mutation of CFTR gene
Cellular Therapy =
injection of material, like epithelial cells, into patient to treat disease
Cells demonstrated CFTR gene:
- achieved through Gel Electrophoresis
Results from a gel electrophoresis test:
Gel electrophoresis technique:
= DNA fragments are separated to determine size
forensics, paternity/maternity tests, DNA sequencing
= determining base sequences of DNA
Chain Termination Method
DNA cut into tiny fragments
Human Genome Project
Predict Gene Function
comparison of unknown gene to similar gene with known function
Determine Evolutionary History
comparison of similar genes between species
= map of person's 23 pairs of chromosomes
What is fluorescence
diagnoses genetic disorders
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