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PCR

PCR
by

Chris Jones

on 16 April 2010

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Transcript of PCR

PCR Polymerase Chain Reaction Polymerase chain reaction (PCR) is used to make millions of exact copies of DNA from a biological sample. DNA amplification with PCR allows DNA analysis on biological samples as small as a few skin cells. With RFLP, DNA samples would have to be about the size of a quarter. The ability of PCR to amplify such tiny quantities of DNA enables even highly degraded samples to be analyzed. Great care, however, must be taken to prevent contamination with other biological materials during the identifying, collecting, and preserving of a sample. 1983 The polymerase chain reaction (PCR) was first conceived by Kerry Mullis, while he was working at Cetus
Corporation. The first paper on the technique was not published until 1985.
1986 The human genetics group at Cetus Corporation, led by Henry Erlich, developed the PCR technique for a number of clinical and forensic applications. This resulted in development of the first commercial PCR typing kit specifically for forensic use about 2 years later. 1986 In People v. Pestinikas, Edward Blake first used PCR-based DNA testing, to confirm different autopsy samples to be from the same person. The evidence was accepted by a civil court. This was also the first use of any kind of DNA testing in the United States.
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