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Let's get to the root of this.

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Harold Moll

on 30 April 2013

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Transcript of Let's get to the root of this.

Blood Agar Morphology


Colonies are pinpoint
0.8-1.2 mm in diameter Gram Stain

gram positive

cocci shape Growth or no growth

negative result Sorbitol Test

negative result Lactose Test

Positive result Enzymatic Activity Sugar fermentation

Phenol Red

negative result alpha-naphthol


Copper or
Red/deep pink

Positive Result arginine dihydrolase

Oil overlay

negative result C Medium
Sucrose and dextrose

Selective and Differential
Crystal violet and potassium tellurite
Trypan blue
Group D and Viridans Group Streptococci

Dextrans Mitis Salivarius Agar (Tellurite) A female patient presents physician's office with slight shortness of breath and minor chest pains,but in considerable distress. A chest X-ray reveals an enlarged heart (endocarditis). The physician on duty explains to the patient that she will need to be admitted, at which point she becomes even more agitated. She has already taken sick leave in the last few days for root canal surgery and, since she has just begun a new job as a Lab Assistant, she is afraid that her company will let her go is she takes any more time off. Harold Moll
DJ Stallworth
Department of Biology
Old Dominion University
General Microbiology Biol 315
April 30, 2013 Let's Get
to the
Root of This 6.5% NaCl Test Mannitol Test Voges-Proskauer Test Arginine Test Glucan Test Medium
Heart Infusion Agar with 5% Sucrose

Non-Selective and Differential
Gram positive cocci that are catalase negative
Group D and Viridans Group Streptococci

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