Loading presentation...

Present Remotely

Send the link below via email or IM

Copy

Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.

DeleteCancel

Make your likes visible on Facebook?

Connect your Facebook account to Prezi and let your likes appear on your timeline.
You can change this under Settings & Account at any time.

No, thanks

Let's get to the root of this.

No description
by

Harold Moll

on 30 April 2013

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Let's get to the root of this.

Blood Agar Morphology

γ-hemolytic
Form-circular
Elevation-raised
Margin-entire

Colonies are pinpoint
0.8-1.2 mm in diameter Gram Stain

gram positive

cocci shape Growth or no growth

negative result Sorbitol Test

negative result Lactose Test

Positive result Enzymatic Activity Sugar fermentation

Phenol Red

negative result alpha-naphthol

KOH

Copper or
Red/deep pink

Positive Result arginine dihydrolase

Oil overlay

negative result C Medium
Peptones
Sucrose and dextrose

Selective and Differential
Crystal violet and potassium tellurite
Trypan blue
Group D and Viridans Group Streptococci

Glucans
Levans
Dextrans Mitis Salivarius Agar (Tellurite) A female patient presents physician's office with slight shortness of breath and minor chest pains,but in considerable distress. A chest X-ray reveals an enlarged heart (endocarditis). The physician on duty explains to the patient that she will need to be admitted, at which point she becomes even more agitated. She has already taken sick leave in the last few days for root canal surgery and, since she has just begun a new job as a Lab Assistant, she is afraid that her company will let her go is she takes any more time off. Harold Moll
DJ Stallworth
Department of Biology
Old Dominion University
General Microbiology Biol 315
April 30, 2013 Let's Get
to the
Root of This 6.5% NaCl Test Mannitol Test Voges-Proskauer Test Arginine Test Glucan Test Medium
Heart Infusion Agar with 5% Sucrose

Non-Selective and Differential
Gram positive cocci that are catalase negative
Group D and Viridans Group Streptococci

Glucans
Levans
Dextrans References

Kazakov, A.. Illustration - Two ambulance cars parking near city hospital building. 123RF
Stock Photos. Retrieved from: http://www.123rf.com/photo_14661857_two-ambulance-cars-parking-near-city-hospital-building.html

Barthorpe, A.L., (2013). Animation apps for scenarios in education. CIMA - From a distance.
Retrieved from: http://cima-fromadistance.blogspot.com/2013/02/animation-apps-for-scenarios-in.html

Anonymous, (2011). Medical test-tube with blood samples ID: 82826395. Pixmac picture
market. Retrieved from: http://www.pixmac.com/picture/medical+testtube+with+blood+samples/000082826395

Department of Veterinary Disease Biology, (2011). Faculty of Life Sciences. University of
Copenhagen, Denmark. Retrieved from: http://pictures.life.ku.dk/atlas/microatlas/food/bacteria/Streptococcus_agalactiae/

Visuals Unlimited, (2013). Staphylococcus aureus Bacteria, a gram-positive coccus that causes
toxic shock syndrome and food poisoning, Gram stain. LM X400. Masterfile Corporation. Retrieved from: http://www.masterfile.com/stock-photography/image/861-03339424/Staphylococcus-aureus-Bacteria-a-gram-positive-coccus-that-causes-toxic-shock-syndrome-and-food-poisoning-Gram-stain.-LM-X400

Anderson, P., (2008). Myxoma chest X-ray. Pathology Education Instructional Resource.
Retrieved from: http://wikidoc.org/index.php/Myxoma_chest_x_ray

Dodson, B., (2012). Flesh-eating bacteria inspire highly selective instant adhesive that won't
stick to fingers. Gizmag. Retrieved from: http://www.gizmag.com/superglue-from-flesh-eating-bacteria/21636/

Hynes, W. (2013). Identification of Gram Positive Cocci Flow Chart. In Nazir Barekzi, (2nd
ed), General Microbiology Laboratory Manual (pp. 80). Norfolk, VA: ODU Biology Department.

Leboffe, M.J., and Pierce, B.E. (2011). Catalase Slide Test. In D. Ferguson, (4th ed.), A
Photographic Atlas of the Microbiology Laboratory. (pp. 64). Englewood, CO: Morton Publishing Co.

Tambe Y., (2006). Hemolyses of Streptococcus spp. (left) α-hemolysis (S. mitis); (middle) β-
hemolysis (S. pyogenes); (right) γ-hemolysis (= non-hemolytic, S. salivarius). Wikepedia.com. Retrieved from: http://en.wikipedia.org/wiki/File:Streptococcal_hemolysis.jpg

Leboffe, M.J., and Pierce, B.E. (2011). Bile EsculinTest Results. In D. Ferguson, (4th ed.), A
Photographic Atlas of the Microbiology Laboratory. (pp. 60). Englewood, CO: Morton Publishing Co.

Kiser, K.M.. 6.5% NaCl Positive. St Louis Community College Clinical Laboratory
Technology. Retrieved from: http://users.stlcc.edu/kkiser/naclp.jpg

Leboffe, M.J., and Pierce, B.E. (2011). Methyl Red Test. In D. Ferguson, (4th ed.), A
Photographic Atlas of the Microbiology Laboratory. (pp. 82). Englewood, CO: Morton Publishing Co.

Leboffe, M.J., and Pierce, B.E. (2011). The Voges-Proskauer Test. In D. Ferguson, (4th ed.), A
Photographic Atlas of the Microbiology Laboratory. (pp. 98). Englewood, CO: Morton Publishing Co.

Leboffe, M.J., and Pierce, B.E. (2011). Decarboxylation Test Results. In D. Ferguson, (4th ed.),
A Photographic Atlas of the Microbiology Laboratory. (pp. 68). Englewood, CO: Morton Publishing Co.

Isenberg, H.D., (2007). Identification of Gram Positive Bacteria. In Lynne S. Garcia, (2nd ed),
Vol. 3. Clinical Microbiology Procedures Handbook (pp. 3.18.1). Washington D.C.: ASM Press.

Zimbro, M.J., Power, D.A.,(2003). Mitis Salivarius Agar Tellurite Solution 1%. Difco & BBL
Manual: Manual of Microbiological Culture Media (pp. 369). Sparks, MD: Becton, Dickinson and Company.

Pfaller, M.A. (1999). Biochemical Characteristics of S. mutans, S. sobrinus, S. salivarius, S.
vestibulus, and S. bovis. In Patrick R. Murray, (7th ed), Vol. 3.Clinical Microbiology Procedures Handbook (pp. 291). Washington D.C.: ASM Press.

Karr, S. (2013). Physiological Characteristics. Steve Karr Home Page. Retrieved from:
http://web.cn.edu/stkarr/physiolo.htm

Dowell, V. R., Jr. and T. M. Hawkins. 1974. Laboratory Methods in Anaerobic Bacteriology. CDC Laboratory Manual. USDHEW C. D. C. Atlanta, GA 30333

NCCLS. Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition. (2004). NCCLS document M22-A3. NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

Vijayaraghavan, R., Veerabathran, Alagappan,Karunakaran, and Vinodh. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 31 Jan. 2006. Web. 28 Apr. 2013. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467921/>

"Infectious Disease Index." Streptococcus Salivarius. MSDS, 21 Apr. 2001. Web. 28 Apr.2013.<http://www.msdsonline.com/resources/msds-resources/free-safety-data-sheet-index/streptococcus-salivarius.aspx>. Questions?
Full transcript