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The Antibacterial Efficacy of Oregano (Oreganum vulgare L.)-Based Soap [MMSU LHS-SC Science Research Thesis]

In partial fulfillment of the requirements in Science Research II, MMSU ILS LHS-SC, Batac, Ilocos Norte
by

Guien Eidrefson Garma

on 6 March 2011

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Transcript of The Antibacterial Efficacy of Oregano (Oreganum vulgare L.)-Based Soap [MMSU LHS-SC Science Research Thesis]

The Antibacterial Efficacy of Oregano (Oreganum vulgare L.)-Based Soap In partial fulfillment of the requirements in Science Research II Guien Eidrefson P. Garma
Mar Louie M. Mayubay
Mari Gay Magdalene A. Opelac Mariano Marcos State University
LABORATORY HIGH SCHOOL - SCIENCE CURRICULUM
City of Batac 2906

March 2011 INTRODUCTION Significance of the Study Background of the Study One of the largest problems in the Philippines is the lack of sanitation and improper hygiene. Although most bacteria/monerans are beneficial, a fraction of them are carriers of sicknesses and are the major cause of skin ailments.

The potentials of Oregano (Oreganum vulgare L.), being one of the most commonly grown and highly valued medicinal plant in the Philippines, therefore, need to be explored as a natural component of soap. Although there are commercially produced soaps in the market, most of them are expensive and are not environment-friendly. Growing concerns about environmental protection and usage of possibly harmful chemicals lead humans to explore alternative source of ingredients for soap that are cheap and at the same time environment-friendly. Statement of the Problem Specifically, this answered the following questions:

1.) What is the feasibility of Oregano plant (Oreganum vulgare L.) juice-extract as a material in the preparation of soap?;

2.)How effective is oregano-based soap when added with the following concentrations of oregano juice-extract:

a.)5 ml of oregano juice-extract/330 ml soap solution
b.)10 ml of oregano juice-extract/330 ml soap solution; and
c.)50 ml of oregano juice-extract/990 ml soap solution?;

3.)How much is the production cost of producing oregano-based soap?; and

4.)Can the oregano-based soap inhibit the growth of bacteria that cause skin ailments? A thorough study of the feasibility and effectiveness of an Oregano-based soap attempted to generate data that will be of great importance to people who are seeking for safe, effective, cost-efficient and environment-friendly means of protecting one’s self against certain types of bacteria. Likewise, the information obtained could also serve as baseline information for future researchers who will delve in a similar study. Furthermore, the result can also benefit private entrepreneurs who are interested to engage in the production of plant-derived or natural soaps. Scope & Limitations This study evaluated the effectiveness and feasibility of oregano-based antibacterial soap. The preparations of soaps were limited to bath soap form at different concentrations of oregano juice-extract. METHODOLOGY Research Method and Design The experimental method was used in the study. Experimental research is a highly controlled procedure in which manipulated treatments or actions from a factor or condition are applied upon another effect of a former product or from a previous set-up of experiment.

PHASE I TREATMENTS:

T1 = 5 ml of oregano juice-extract/330 ml soap solution
T2 = 10 ml of oregano juice-extract/330 ml soap solution
T3 = no oregano juice-extract (Control)

PHASE II TREATMENTS:

T1 = pure oregano juice-extract (Control)
T2 = 50 ml of oregano juice-extract/990 ml soap solution Materials and Equipment Vegetable oil
Water
Solid NaOH (caustic soda)
Oregano extract
Staphylococcus aureus
Pseudomonas aeruginosa
Escherichia coli
MRSA
500 ml beakers
1000 ml beaker
Graduated cylinders
Wooden and plastic serving spoons
Molder RESULTS & DISCUSSION Soap Production Extraction of oregano juice/extract
200 g caustic soda per 500 ml water
660 ml vegetable oil per 330 ml caustic soda solution
Soap production according to treatments Antibacterial Tests Swabs from soap dilutions
Saturation of blank paper discs
Culturing of bacteria in petri dishes
Strains used:
-S. aureus
-MRSA (Phase I only)
-P. aeruginosa
-2 strains of E. coli
24-hour incubation SUMMARY, CONCLUSIONS & RECOMMENDATIONS CONCLUSIONS:
Oregano as a base ingredient for antibacterial soap has antibacterial properties that could inhibit growth of various disease causing bacteria but only at certain limitations and only when prepared at higher concentration.
The inhibitions of bacterial growth only at certain zones indicate that bacterial growth inhibition was very minimal.

RECOMMENDATIONS:
Oregano-based antibacterial soap be prepared at higher concentration in order to enhance its antibacterial properties.
Same experiment be conducted with higher concentrations of oregano extract and with careful procedure in the preparations of the materials in order to avoid contaminations of the experiment.
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