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Lab Safety

Personal Protective Equipment

  • Glasses vs. goggles
  • Gloves
  • Lab coat
  • Face shields

Location & Function of:

  • Gas shutoffs
  • Fire extinguishers
  • Fire blankets
  • Eyewash/shower

Lab Equipment

Serological and Pasteur pipets

Washing Glassware:

  • Add small amount of laboratory soap and use a brush to make sure all surfaces are washed
  • Rinse with tap water until no suds are evident, usually at least 3 times
  • Rinse twice with distilled water
  • Air-dry or use oven to dry

Numerical Data

Universal Hazard Label

Erlenmeyer Flasks

  • error ~ 5% from target volume

Graduated Cylinders

  • In general, larger = less accurate volume measurement due to large diameter of the meniscus

motorized pipet pump

tri-valve pipet

manual pipet pump

Pipet Pumps

Autoclave

Disposable Plastic Pipets

Reagent Bottles

Micropipets

Electric Scales

Weigh Boats

Burettes

Labeling

  • Name of solution
  • Concentration
  • Date made
  • Name of person who made it
  • Potential hazards if necessary

Biotechnology

Laboratory Skills

Scientific Notation

  • Shorthand method to display numbers that convey their level of precision
  • Uses a number that is multiplied by 10 raised to a positive or negative number

Significant Figures

Rule 1: The number of significant figures is the number of digits to the right of the first non-zero number.

Rule 2: When adding or subtracting numbers, the resulting numbers cannot have any more significant figures to the right of the decimal point than any value used in the calculation.

Rule 3: When multiplying or dividing numbers, the answer cannot have any more significant figures than the value in the calculation with the fewest significant figures.

Percent Solutions

Mass per Volume (m/v)

Preparing Solutions

A Stock Solution is a concentrated solution that will be diluted to some lower concentrated for actual use. Stock solutions are used to save preparation time, conserve materials, reduce storage space, and improve the accuracy with which working lower concentration solutions are prepared.

Stock Solution

Dilutions

Volume per Volume (v/v)

Molar Solutions (Proportional Method)

vs.

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