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Transcript of Physio
-it acted on the respiratory and cardiovascular systems. Group 2 EXPERIMENT III
INDEPENDENT IRRITABILITY OF MUSCLE Candelario, Jorgina
Lopez, Andrea Joyce
Manalo, Frances Anne
Sicat, Camille Use a whole frog for this experiment Do not pith nor cut off the head of the frog. Connect in a series of fry cell, simple key and inductorium for single make and break shocks. Connect a hand electrode to the inductorium.
Kymograph record not needed. Surgical Procedures: Incision
Isolate sciatic nerves w/o injuring accompanying blood vessels.
Place a tight ligature around the left thigh.
Exclude the nerve from the ligature.
Expose the gastrocnemius muscle. Experimental Procedures: Stimulate each nerve and gastrocnemius w/ threshold stimulus. Note the response.
Inject 0.3 cc lidocaine into ventral lymph sac.
Wait for 5 minutes.
Stimulate both nerves and muscles using the same threshold as before. Results: Before Injection After Injection left nerve
right muscle - - + + - - - - 1. Lidocaine
-is a common local anesthetics amd anti arythmic drug.
-works by stoping nerves from sending pain signals. 2. How will injuring the accompanying blood vessels affect the results of your experiment?
-it will cause hemorrhage
-interruption of blood flow
-increase tendency w/in blood to form clots
-blood vessel injury 3. Explain your results. Draw conclusions. 5. Independent irritability of muscle. 6. Mechanism of conduction of impulse at the neuromuscular junction.
-AP travels down the motorneuron to the presynaptic terminal.
-Depolarization of the presynaptic terminal opens Ca2+ channels, and Ca2+ flows into the terminal
-ACh is extruded into the synapse by exocytosis.
-ACh binds to its receptor on the motor end plate.
-Channels for Na+ and K+ are opened in the motor end plate.
-Depolarization of the motor end plate causes AP to be generated in the adjacent muscle tissue.
-ACh is degrade to choline and acetate by AChE, choline is taken back into the presynaptic terminal on Na+ choline co transporter. This means that muscles are independent from reacting to the stimulus. They are independent from nerve.