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The basics of chromatography for A Level Chemistry

Cycling Scientist

on 1 May 2011

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Transcript of Chromatography

Chromatography Separation of a mixture Analysis Stationary Phase
Mobile Phase Thin Layer Chromatography HPLC GLC GCMS Column Chromatography Has my reaction finished? Is my product pure? What is my product? We can use TLC to follow a chemical reaction
if the reactants and products behave differently
under chromatography. Chromatography can show you when a
substance is impure, but we can never
show that it is pure. Why not? If the compound is known we can compare
its behaviour under different chromatography
conditions with a reference sample. If it behaves similarly under ALL
conditions what can we deduce? In Organic Chemistry, TLC is often used. Once a mixture has been resolved
by TLC we can use column chromatography
to separate the components. Watch the video to see how this works. When attached to the mobile phase
the molecules move relative to the
stationary phase. The mobile phase is either
liquid or gaseous When the molecules are attached to the
stationary phase
they do not move The stationary phase is either
a solid or a viscous liquid. Watch the videos to see how this works. Equilibrium The substances are all each
in equilibrium between the
mobile phase and the
stationary phase. This determines how quickly
each component move through the mobile phase It is therefore likely that different
chemical substances will move at
different rates through the
stationary phase... The video is comprehensive and would enable
you to carry out a "professional" separation, but
it demonstrates the technique very well. It is also 20 mins long! Watch the video to see
how this works Watch the video to find out how
HPLC is carried out. This video outlines mass spec, but towards the end (approx 6:40 in) it shows how GC-MS works. A technique for separating mixtures that has a multitude of guises and methods but always utilises the different solubility of the components in two "phases", one mobile and one stationary. ...as they are individually conveyed
by the mobile phase ...which will effect a separation that can be used for... Instrumental methods In Summary
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