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Commando-ROBO

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by

Rachel Yang

on 19 August 2013

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Transcript of Commando-ROBO

Prostate Cancer Stimulated by AR
What effect will Docetaxel have on AR expression?
Supressing the AR with siRNA
Combining ASC-J9 with Docetaxel
Docetaxel (chemotherapy)
Casodex (anti-androgen)
MDV3100 (2nd generation)
C4-2
0 nM
0.1 nM
0.25 nM
0.5 nM
1 nM
2 nM
4 nM
8 nM
Western Blot
qPCR
siRNA
MTT Assay
MTT Assay
GAPDH
0 nM Docetaxel
2 nM
Docetaxel
4 nM
Docetaxel
8 nM
Docetaxel
16 nM Docetaxel
32 nM
Docetaxel
MTT Assay
C4-2
C4-2
AR
GAPDH
docetaxel (nM)
0 nM
32 nM
2 nM
4 nM
8 nM
16 nM
12µl
20µl
20 µl
C4-2
AR
GAPDH

docetaxel (nM)
0 nM
2 nM
4 nM
8 nM
16 nM

8 nM

32 nM

qPCR
Western Blot
0 nM
0.1 nM
0.2 nM
0.5 nM
1 nM
2 nM
3 nM
4 nM
0 nM
0.1 nM
0.2 nM
0.5 nM
1 nM
2 nM
3 nM
4 nM
docetaxel (nM)

docetaxel (nM)

C4-2
docetaxel (nM)

AR
GAPDH
0 nM
0.1 nM
0.1 nM
0.2 nM
0.5 nM
1 nM
2 nM
3 nM
4 nM
1 D
12 hr CHX

1 D
6 hr CHX

1 D
4 hr CHX

1 D
2 hr CHX

1 D
1 hr CHX

1 D (B)
cntrl
12 hr CHX

cntrl
6 hr CHX

cntrl
4 hr CHX

cntrl
2 hr CHX

cntrl
1 hr CHX

cntrl (B)
1 nM Docetaxel
Control
GAPDH
AR
time (hr)
12 hr CHX
6 hr CHX
4 hr CHX
2 hr CHX
1 hr CHX
CHX (B)
12 hr CHX
6 hr CHX
4 hr CHX
2 hr CHX
1 hr CHX
CHX (B)
1 nM Docetaxel
Control
AR
GAPDH
time (hr)
12 hr CHX
6 hr CHX
4 hr CHX
2 hr CHX
1 hr CHX
CHX (B)
12 hr CHX
6 hr CHX
4 hr CHX
2 hr CHX
1 hr CHX
CHX (B)
Full transcript