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LAB 2

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by

piero carletti

on 3 December 2012

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Transcript of LAB 2

Synthesis and Evaluation of Artificial Kidney Stones 1 Introduction 2 Objective 3 Methods 4 Results 5 Conclusion 1 Introduction What are kidney stones? crystals or rocks that form due to the clumping of minerals found in urine. 2 Objective The purpose of this experiment was to devise a method of synthesizing the main types of kidney stones existing to perform further physical and chemical analysis on them. 3 Methods Calcium Oxalate
Struvite
Calcium Phosphate Calcium Oxalate Mix both reactants in equimolar conditions in 200mL of DI water (0.025mol)
centrifuge for 5 minutes and wash the pellet twice centrifuging each time
Weight the pellet
Add EDTA and observe Struvite Mix all reactants in equimolar amounts in 200mL DI water (0.025mol)
Add 10mL of pH 10 buffer
centrifuge for 5 minutes and wash the pellet twice centrifuging each time
Weight the pellet
Add EDTA and observe Calcium Phosphate 4 Results Types of Stones There are five types of stones. Calcium Oxalate
Calcium Phosphate
Uric Acid
Struvite
Cystine Reactants: Mix both reactants (0.02mol and 0.03mol)
Add 10mL of pH 10 Buffer
centrifuge for 5 minutes and wash the pellet twice centrifuging each time
Weight the pellet
Add EDTA and observe Calcium Oxalate
Struvite
Calcium Phosphate Calcium Oxalate Struvite Calcium Phosphate 5 Conclusion Kidney stones as a common health issue

Possible sources of errors

Addition of EDTA and its effect on solubility and Le Chatelier's principle.
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