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Brine Shrimp Experiment

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Dakotah Watson

on 7 March 2013

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Transcript of Brine Shrimp Experiment

Brine Shrimp Experiment Carolina Natural Selection for AP Biology The question we were answering: Does darkness, such as flooding of a lake or ocean to create a deeper area, affect the hatching viability of Brine Shrimp? Our Hypothesis: If there are two colonies of Brine Shrimp, one in darker waters the other in lighter waters, then the Brine Shrimp in darker waters are less likely to hatch and survive compared to the Brine Shrimp in lighter waters. The Experimental Procedure 1. Pour 30 Milliliters of Saline Solution in to a petri dish, in this experiment, we used 6 at 2%.
2. Put double-sided tape on to a microscope slide, in this experiment, we used 6.
3. Take a paint brush and dab it into the Sea Monkey eggs.
4. Put the eggs onto the tape on the slide.
5. Put the slide underneath the microscope.
6. Count how many eggs are on the slide, aiming to reach near 20 to 30 eggs per slide. The Experimental Procedure
(cont.) 7. Put the slides into the water, one per petri dish.
8. Now, take aluminum foil and wrap your experimental groups. In this experiment, we had two dishes completely covered, two halfway covered, and two completely uncovered.
9. Place the petri dishes out of the way, in a safe area for 24 hours. The Experimental Procedure
(Cont.) 10. Count how many shrimp have hatched, are partially hatched, and have not hatched at all. Record your results.
11. Repeat at 48 hour mark.
*Remember, if you have any swimming, pick them up in a pipet and squirt them into a larger container with the same solution. You will put all the ones swimming into the same container. Conclusion
(Cont.) Our hypothesis was supported by this experiment because more Brine Shrimp hatched in the completely uncovered dishes, than those in the completely covered, or half covered dishes. If you were to redo this experiment: Would you change your hypothesis (and how)? NaCl% Hatching Viability
0 0
0.5 15
1 22.5
1.5 16.6
2 48.4 What did you find out? 48 hours 24 hours 0 hours Results- Graph Conclusion Did your experiment fail to support or support your hypothesis? Yes, our experiment supported our hypothesis Problems 1. Leaks caused by aluminum foil
2. Inaccurate counting
3. Salt measurement
4. SABOTAGE!!!!!!!!
5. Inability to collect all swimmers.
6. Spills What sources of Potential Error occured? Problems (Cont.) Did any other questions arise as the result of your experiment? 1. Does Aluminum Foil cause Petri dishes to leak?
2. Is there another way to cover the Petri dishes without using Aluminum Foil?
3. Could Duct Tape be an alternative? If you were going to redo this experiment: would you change anything (and how)? No, we would not change our hypothesis.
Why change something when it doesn't need changing? :) We would only change what we would use to cover the petri dishes and make our Sea Monkeys Revolt. Results- Table Variables Independent Our independent variable was the amount of light in which the Brine Shrimp were introduced. Variables Dependent Our dependent variable was the number of shrimp that hatched. Will they survive afterall?
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