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Lab 3 - Identifying Macromolecules

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Angela Martinez

on 25 October 2012

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Transcript of Lab 3 - Identifying Macromolecules

+/- Test Lugol's Test for Starch Sudan Test for Lipids Blot Test for Lipids The Experiment! Lab #3 +/- Test Benedict's Test for Reducing Sugars Biuret Test for Protein By Angela, Carl, Jessica, and Megan Identifying Macromolecules The Introduction 4 Macromolecules:
Nucleic Acids
Lipids October 25, 2012 5 Tests:
Lugol's Iodine
Benedict's Solution
Biuret Test
Sudan Stain &
the Blot Test The Discussion! Other Ingredients: Proprietary Protein Blend (Whey Protein Concentrate, Whey Protein Isolate, Hydrolyzed Whey Peptides), Cocoa, Artificial Flavor, Lecithin, Salt, Acesulfame Potassium, Cellulose Gum, Xanthan Gum Experimental Results The Color Wheel Benedict's Why it Works! Chromatography With Unlimited Resources... Future Experiments Materials Nutritional Shake
Benedict's Solution
Lugol's Solution
Sudan Solution
Biuret Solution
Paper Towels
Test Tubes
Distilled Water Olive Oil
Cow Albumin
1% Glucose Solution
1% Starch Solution
Prior results to make a comparison to The Results! Whey Chocolate Shake (GNC) Macromolecules Present (Y/N) Carbohydrates
Proteins Yes
Yes Oxidized Concentration of sugars increases the intensity of the solution carbonyl Lugol's Solubility change Sudan Test Depends on solubility Biuret Test NMR Mass Spectrometer Spectrophotometer Mix 1% glucose with Benedict's solution
Place in hot water bath for 5 mins.
Set aside + Mix water with Benedict's solution
Place in hot water bath for 5 mins.
Set aside - Variable Test Mix nutrition shake with Benedict's solution
Place in hot water bath for 5 mins.
Compare to +/- tests Variable Test + - Mix 1% starch solution with Lugol's solution
Set aside Mix water with Lugol's solution
Set aside Mix nutrition shake with Lugol's solution
Compare to +/- tests +/- Test Variable Test + - Mix half olive oil and half water with sudan stain
Shake well and set aside Mix water with sudan stain
Shake well and set aside Mix half nutrition shake with half water with sudan solution
Shake well
Compare to +/- tests +/- Test + - Variable Test (No test done) Place 5 drops of olive oil on a paper towel
Let sit until dry
Set aside Place 5 drops of water on a paper towel
Let sit until dry
Set aside +/- Test Mix cow albumin with biuret
Shake and set aside + Mix water with biuret
Shake and set aside - Variable Test Mix nutrition shake with biuret
Shake and compare to +/- tests Benedict's
(sugar) Lugol's
(starch) Sudan
(lipid) Blot Test
(lipid) Biuret
(protein) Positive Control Negative Control Nutrition Shake Biomolecule Present? orange/brown light blue (no change) tan/darker brown yes black amber black yes top = orange solid
bottom = pink liquid pink liquid tan/pink liquid no darkened paper no change (no test) --- light purple clear (no change) darker brown yes The End! Thank you! Citations http://www.gnc.com/product/index.jsp?productId=2944029 (nutritional information)
http://www.suzannecooper.com/classroom/colorwheel.html (color wheel)
http://chemnmr.colorado.edu/moreinfo/instruments/300NMR.html (NMR pic)
http://www.faqs.org/espionage/Nt-Pa/Nuclear-Spectroscopy.html#b (NMR info)
http://www.biosci.ohiou.edu/introbioslab/Bios170/170_2/benedict.htm (Benedict’s Test)
http://www.webexhibits.org/causesofcolor/6AC.html (Lugol’s Test)
http://www.baruch.cuny.edu/wsas/departments/natural_science/biology/bio1003/organic.html (Sudan Test)
http://www.brilliantbiologystudent.com/biuret_test.html (Biuret Test)
http://alevelnotes.com/Carbohydrate-polymers/65 (Starch Image)
http://www.orcagrowfilm.com/Articles.asp?ID=145 (Visible Light Spectrum)
http://www.panoramio.com/photo/6833871 (Beer Factory Picture)
http://tainano.com/chin/Molecular%20Biology%20Glossary.htm (Chromatography)
http://www.pittstate.edu/department/chemistry/nmr-instruments.dot (NMR)
http://www.lamondlab.com/MSResource/LCMS/MassSpectrometry/massSpectrometry.php (Mass Spec)
http://www.cnrinch.com/vis-spectrophotometer.htm (Spectrophotometer)
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