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Photosynthesis Lab Mini-Poster
Transcript of Photosynthesis Lab Mini-Poster
Materials and Methods
<The Effect of Boiling the Chloroplasts on Photosynthesis >
Include all published works mentioned in your presentation. List in APA bibliographic form.
How does boiling the chloroplasts affect their photosynthesis?
Boiling the chloroplasts will have no effect on their rate of photosynthesis. As time, the independent variable, is increased, the dependent variable, percent of light absorbed, will increase too at the same rate, for both the control and the experimental groups.
Behm, N. (2011). Retrieved from http://www.tiffin.k12.oh.us/userfiles/987/Classes/1553/
Percent of light absorbed at each time interval (in minutes) Trial One
Time Elapsed (minutes)
Inorganic molecules are changed into organic molecules for life's energy use through photosynthesis. Photosynthesis is largely performed in plants by their chloroplasts. The chloroplasts convert light energy, water, and carbon dioxide into the oxygen and sugar that is needed for cellular respiration. However, the proteins and enzymes in the chloroplasts that are vital to the photosynthesis process are susceptible to denaturing when exposed to high levels of heat, just like any other protein.
Campbell, N., Reece J., Taylor, M., & Simon, E. (2006). Biology: Concepts and Connections.
(5th ed.). San Francisco, CA: Pearson Custom Publishing.
6 mL Distilled Water
Spectrophotometer to measure the light absorbed
2 mL DPIP
3 oz spinach leaf circles
3 oz boiled spinach leaf circles
***In the interest of safety, use gloves when handling DPIP. Use spectrophotometer as intended. Keep all materials out of mouth and eyes.***
Use one blank cuvette to set the spectrophotometer. Put 3 mL of water into each of the remaining two cuvettes (cuvettes 1 and 2). Put 1 mL of DPIP into each of those cuvettes. Add non-boiled leaves to cuvette 1 and boiled leaves to cuvette 2. Place both cuvettes under light source. Place each cuvette into the spectrometer every 5 minutes for 20 minutes to read the percent of light absorbed. Repeat trial twice more.
The effect of boiling the chloroplasts on photosynthesis is measured by placing boiled and non-boiled leaf circles inside cuvettes with water and DPIP under a light source for 20 minutes, measuring the percent of light absorbed every 5 minutes with a spectrophotometer. The non-boiled leaves will act as the control group. The expected result is that the boiled leaves will absorb very little light, showing that little photosynthesis is being performed, because the proteins in the chloroplasts will have denatured and stopped functioning. This result occurred, with the non-boiled chloroplasts doubling the percent of light absorbed during the experiment, while the boiled chloroplasts showed almost no change in light absorbed.
This experiment tested whether the boiling of chloroplasts would have any effect on their photosynthesis by testing how much light was absorbed by boiled chloroplasts and non-boiled chloroplasts. The null hypothesis, which stated that boiling the chloroplasts would have no effect on the rate of photosynthesis, was falsified. The expected result-- that the boiled chloroplasts would have dramatically lowered photosynthesis activity--occurred. The control group of non-boiled chloroplasts more than doubled the percent of light absorbed, while the boiled chloroplasts absorbed very little light. This is most likely due to the boiling of the chloroplasts having exposed them to high enough temperatures that the heat denatured the proteins inside the chloroplasts. Since the proteins lost their shapes, they lost their functions, stalling the completion of their "jobs" and inhibiting the entire process of photosynthesis.
Further research can be conducted on this subject by conducting experiments to see, as the temperature is increased, at what exact temperature do the chloroplasts stop functioning.
Independent Variable: Time Dependent Variable: Percent of light absorbed
Central Virginia Governor's School for Science & Technology. (n.d.). [photo of cuvette being
inserted into spectrophotometer]. Retrieved from http://www.cvgs.k12.va.us:81/curric
Trial One Percent of light absorbed over time
General Botany Laboratory BYUI. (2011). [photo of leaf discs and hole puncher] Retrieved
Joiner, S. (n.d.). Retrieved from http://www.scribd.com/doc/30834264/AP-Lab-4
Luu, D. (n.d.). Retrieved from https://sites.google.com/site/dexterluusaplabs/ap-lab-4