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Cellular Respiration Lab

by Paige, Sarah, Hayleigh, and Megan
by

Megan Bennink

on 4 January 2013

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Transcript of Cellular Respiration Lab

Abstract Introduction: Cellular Respiration Lab Methodology 1) Make the phenolphthalein solution
1) Fill a 200 mL beaker with 50 mL of distilled water
2) Using a pipette put three drops of phenolphthalein into the distilled water
3) Using a different pipette put three drops of sodium hydroxide into the distilled water
4) Use a straw to stir the mixture (should be pink) Literature Cited Campbell, Biology, 8-e AP

www.wikipedia.com

cellularrespiration.net Statistical
Analysis Discussion After collecting data from this lab, we found that it seems to be that with an increase in heart rate, cellular respiration also increases based on Quentin's results. This supports our hypothesis because the higher his heart rate, the more CO2 is given off. The way that we tested to see how much CO2 was given off, was by using phenolphthalein to have a visual to be able to time to see how quickly the solution turned from pink to clear. If it took only a short period of time to get the solution to change from pink to clear, that means that more CO2 was given off. However, our data was overall inconclusive and more tests would have to be performed in order to get better results. One way to improve the lab and assure that there is more accurate data is to use a heart rate monitor and a respirometer. The use of these two tools would allow for data that is much more accurate, and would give better results. Another improvement could be to make sure that both people who are respiring through the straws are breathing at the same rate, otherwise the solution may turn clear sooner for one person, regardless of their heart rate. An additional problem that we may have had in our lab is that we did not allow for the heart rate return to resting rate. This may have affected the data we collected, and if we were to have let the heart rate go back down we may have had more conclusive data. by Paige, Sarah, Hayleigh, and Megan Background: Cellular respiration is a set of metabolic reactions that convert energy taken in from oxygen and glucose from food into energy for the organism in the form of ATP while CO2 and water are produced as a result. CO2 given off as a waste product. There are multiple ways one can measure the rate at which cellular respiration occurs. For example, CO2 and Oxygen probes can give you a calculation of the rate at which it is going with numbers or quantitative data. In our experiment however, we will be using a different method. Phenolphthalein is a chemical compound that is colorless in an acidic solution and turns pink in a basic solution. To use this in a cellular respiration experiment, this helps us see how quickly an organism is giving off CO2, which makes the solution more acidic turning the once pink solution to clear. To make the solution basic to begin with, we used sodium hydroxide in water. We know that when an individual exercises, their heart rate goes up and they are using more energy.

Purpose: We want to find out if heart rate has any correlation to the rate at which cellular respiration occurs. And if so, what that correlation is.

Hypothesis: If we measure CO2 levels given off after various exercise levels, then the higher the pulse rate, the more CO2 given off. Materials Procedure 2) Mix up one solution per test subject
3) Place the bottom edge of a piece of tape on the 150 mL mark on each beaker (when the test subjects blow into the solution the bubbles should not pass this mark)
4) Take the heart rate of the test subjects (heart beats per minute)
5) Give each subject a straw and a beaker of solution
6) Have a person time each test subject with a stop watch (starts the clock when they blow bubbles stopping when the solution turns clear) -remember step three- 7) Have another person watch a clock and tell the test subjects to blow into the solution for five sec. and breath in for 2 sec. (keep alternating till the subjects have both their solutions clear)
8) Record data
9) Have the test subject increase their heart rates by doing exercise (we used stairs)
10) Repeat steps 1-9 for more trials Phenolphthalein
Straws
200 mL beakers
Distilled water
Sodium Hydroxide
Pipettes
Stopwatch
Clock
Tape The purpose of the experiment was to determine the affects heart rate has on cellular respiration. Two test subjects took their resting heart rate and their Carbon Dioxide release level, then were made to exercise a set amount. After exercising, they took their heart rate and were made to blow into a solution made of phenolphthalein, distilled water, and sodium hydroxide. This measured their Carbon Dioxide release level. There were outliers for both are test
subjects. For the female there were
two results that did not fit the trend.
The other three were also questionable.
For the male the results had a better
trend, even though there was one
strong outlier. We found the line of best
fit for the two sets of data. To find the
males line of best fit we removed the
outlier. We found that they had a
negative trend. This means that more
CO2 was released as the heart rate
went up. Megan Quentin age: 17
height: 5'11'' Megan is a senior at Allendale
High School. She enjoys swimming, drawing and hunting. age: 17
Height: 5' 9" Quentin is also a senior at Allendale
High School. He enjoys playing
basketball, hanging out with his
friends and giving Paige a hard time
everyday of her life. Favorite type of Bean: Jelly Beans Favorite type of Bean: Jumping Beans
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