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Microbiology LAB Final Presentation Group 5
Transcript of Microbiology LAB Final Presentation Group 5
GROUP 5 2013/1/9 Culture Transfer Aseptic techniques are used doing subculture.
sterilize inoculating instruments in the flame.
Prepare and maintain stock cultures, as well as in microbiological test procedures. Isolation of Pure Cultures Streak-plate method.
Pure culture: contain only one type of organism, for the study of their cultural, morphological, and biochemical properties.
Colony: individual ,the multiplication of a single organism. Simple Staining To determine the morphology and arrangement.
A single reagent.
Basic stains: methylene, crystal violet, carbol fuchsin. Gram Stain Differential staining.
1. Primary stain.
2. Decolorizing agent.
3. Counterstain. Gram-positive & negative
The different composition of cell walls.
(+) thick peptidoglycan layer
(-) thin peptidoglycan layer Spore Stain Life cycle of a spore-forming bacterium. Primary Stain: Malachite Green
Decolorizing agent: Water
The spore remains green. Differential, Selective & Enriched Media Specialized media for the selection and differentiation of microorganisms. Enriched Media Media with highly nutritious materials.
Blood, serum, yeast extract......
For the cultivation of fastidious microorganisms. BLOOD AGAR What is qPRC?? The blood is an enrichment ingredient.
1. Gamma hemolysis
2. Alpha hemolysis
3. Beta hemolysis Real-time PCR "Quantitative" real- time PCR:focus on.........
The process of qPCR:
1.cDNA is reverse transcripted from mRNA.
2.The PCR is prepared as usual....
3.Resulting in an increase in fluorescence
4.Fluorescence is detected and measured. A DNA-binding dye Intercalating dyes Probe-based detection Selective Media Isolate specific groups of bacteria.
Substances that (-) the growth of one type of bacteria but (+) another. Phenylethyl alcohol agar
(-) gram-negative Crystal violet agar
(-)gram-positive 7.5% NaCl agar
(+) halophilic microorganisms Differential/ Selective Media Chemical compounds that produce a characteristic change in the appearance of bacterial growth or the medium surrounding their colonies. Mannitol salt agar
Fermenter: yellow zone
Nonfermenter: no change MacConkey agar
Fermenter: red on the bacterial surface;
E.coli: pink medium Nonfermenter: transparent Eosin-methylene blue agar
dyes: eosin & methylene blue
(+) gram-negative, (-) gram-positive
Fermenter: thick, mucoid, pink colonies
E.coli: blue-black with a metallic green sheen
Nonfermenter: transparent Reference THE END :)
Thank you for your listening!! 1.Google big god's picture
2.Microbiology: A Laboratory Manual: International Edition, 9/E
4.Science Photo Library 宮心計