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Green Chemistry Project

Extraction of Lycopene from Tomato Paste

bella sarjoh

on 24 April 2011

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Transcript of Green Chemistry Project

Extraction of Lycopene from Tomato Paste Green Chemistry Project Bella Sarjoh Green Chemistry Project Extracting Lycopene from Tomato Paste Bella Sarjoh Lycopene C40H56 A bright red caretenoid pigment that's found in tomatoes and other fruits and vegetables. Lycopene is a polyunsaturated hydrocarbon. It has eleven conjugated double bonds that gives it its deep red color, and is responsible for antioxidant activity. Different sudies have suggested that lycopene because of its antioxidative properties reduces the risk of cancer, and chronic disease from chronic stress Introduction In this experiment Lycopene was extracted from tomato paste by washing with different amounts of acetone, petroleum ether, saturated sodium chloride solution and 10% potassium carbonate solution. It was then separated from the other pigments present in the product using TLC with three different mixtures of ethyl acetate and hexane. A mixtureof acetone and hexane was used because acetone is soluble in water where hexane is not. So at room temperature all the pigment is transferred to the hexane layer, making separation easier. Saturated sodium chloride is used inorder to saturate and therefore properly separate the two layers formed. Potassium carbonate was added in order to get rid of the natural acids from the tomato this was accompanied with the release of gas.
Green Project Green chemistry, also known as sustainable chemistry, is the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances. This experiment is green because of the isolation of natural product, and no chemicals that are harzadous to the environment were used in the extraction or separation process. Procedure Results Procedure:
Starting this lab consisted of gathering all necessary materials for both the extraction and separation.
After all equipment was gathered one gram of tomato paste was measured out and collected in a 15-mL vial. Four milliliters of a 50% acetone and petroleum ether solution was then added to the tomato paste to dissolve the pigments in solution.
The aqueous (top) layer was removed and washed with five milliliters of a saturated NaCl solution, followed by 5 milliliters of 10% K2CO3, and lastly another five milliliters of saturated NaCl solution.
The bottom (aqueous) layers were removed for all three of the washes. Differences between the aqueous layers and the organic layers were obvious with the aqueous layer being orange-yellow.
The mixture of now pure pigments in solution was then placed in the rotavap and evaporated. This was then used to do the TLC separation. Other than the addition of hexanes, the pigments were able to be separated based on their differences in polarity and size.
Results 1:2 (Ethyl Acetate : Hexane)0.98
1:3 (Ethyl Acetate : Hexane)0.98 Peaks 2930 2860 1620
CH3 (alkanes) CH2 (alkanes) C=C (alkenes) Rf Values Summary Reference The presence of Lycopene was confirmed in after analyzing the IR spectrum and confirming the presence of all expected groups. The separation from the TLC also confirmed the presence of three pigments as was expected http://www.naturalmedicinejournal.com/article_content.asp?edition=1&section=2&article=120 http://www.molecularshirts.com/index.php?module=pagemaster&PAGE_user_op=view_page&PAGE_id=9&MMN_position=14:14 http://www.lycocard.com/index.php/lyco_pub/health/ Sources: Summary: The End
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