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Green Chemistry Project
Transcript of Green Chemistry Project
Green Project Green chemistry, also known as sustainable chemistry, is the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances. This experiment is green because of the isolation of natural product, and no chemicals that are harzadous to the environment were used in the extraction or separation process. Procedure Results Procedure:
Starting this lab consisted of gathering all necessary materials for both the extraction and separation.
After all equipment was gathered one gram of tomato paste was measured out and collected in a 15-mL vial. Four milliliters of a 50% acetone and petroleum ether solution was then added to the tomato paste to dissolve the pigments in solution.
The aqueous (top) layer was removed and washed with five milliliters of a saturated NaCl solution, followed by 5 milliliters of 10% K2CO3, and lastly another five milliliters of saturated NaCl solution.
The bottom (aqueous) layers were removed for all three of the washes. Differences between the aqueous layers and the organic layers were obvious with the aqueous layer being orange-yellow.
The mixture of now pure pigments in solution was then placed in the rotavap and evaporated. This was then used to do the TLC separation. Other than the addition of hexanes, the pigments were able to be separated based on their differences in polarity and size.
Results 1:2 (Ethyl Acetate : Hexane)0.98
1:3 (Ethyl Acetate : Hexane)0.98 Peaks 2930 2860 1620
CH3 (alkanes) CH2 (alkanes) C=C (alkenes) Rf Values Summary Reference The presence of Lycopene was confirmed in after analyzing the IR spectrum and confirming the presence of all expected groups. The separation from the TLC also confirmed the presence of three pigments as was expected http://www.naturalmedicinejournal.com/article_content.asp?edition=1§ion=2&article=120 http://www.molecularshirts.com/index.php?module=pagemaster&PAGE_user_op=view_page&PAGE_id=9&MMN_position=14:14 http://www.lycocard.com/index.php/lyco_pub/health/ Sources: Summary: The End