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Anti-fungal Property of Lemongrass (Cymbopogon Citratus) oil

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Carla Bato

on 13 August 2015

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Transcript of Anti-fungal Property of Lemongrass (Cymbopogon Citratus) oil

Lemongrass (Cymbopogon Citratus)
Statement of the Problem
Hypothesis
There is no significant difference between the zones of inhibition of the Trichophyton rubrum after applying the Lemongrass oil and the control, Ketoconazole
2. What concentration of extracted Lemongrass oil will produce the largest zone of inhibition against Trichophyton rubrum?
Statement of the Problem
3. Is there a significant difference in the zone of inhibition of the Trichophyton rubrum after applying Lemongrass oil and the control, Ketoconazole?
Statement of the Problem
1. What is the zone of inhibition of T. rubrum after impregnating the disk using different concentrations?
a) 100%
b) 75%
c) 50%
d) 25%
Anti-fungal Property of Lemongrass (Cymbopogon Citratus) oil extract against
Trichophyton rubrum

Presented by:
Cervana, James Matthew
Anteza, Erianne
Batiles, Jomalyn
Bato, Carla
Bayaban, Alissa
Denaga, Marianne
Suarez, Charlene

Our Study is about.....
The zone of inhibition of
Trichophyton rubrum
after embedding the disk using different concentrations
Table 1. Mean of the zone of inhibition the different concentraions
Results and Discussion
Instrumentation
Lemongrass
Mineral oil (Diluent)
Ketoconazole
Purchasing of Lemongrass from supplier in Las Pinas City Market
Preparation of equipment and apparatus needed for the extraction of the Lemongrass
Extraction of the Lemongrass using Hydro Distillation
Dilution of Lemongrass oil extract in different concentrations; 25%, 50%, 75% and 100% using mineral oil as diluent
Preparation of Potato dextrose agar incorporated with a culture
Trichophyton rubrum
Application of the standard, Ketoconazole and the different concentrations Lemongrass oil extract in filter paper disc
Embedding of the filter paper disc immersed in Ketoconazole and the four concentrations of Lemongrass oil Extract on the culture media in triplicate plates
Incubation of plates for 7 days
Measure the zone of inhibition
Lemongrass Oil Extract
Methodology
Concentration of extracted Lemongrass oil that produced the largest zone of inhibition
Table 2. Mean of the Zone of inhibition of the standard and different Lemongrass Concentrations for the 4th Day
Table 3. Mean of the Zone of inhibition of the standard and different concentrations for the 7th Day
Difference between the zones of ihibition of
Trichophyton rubrum
after application of Lemongrass oil extract and the standard, Ketoconazole
Summary, Conclusion and Recommendations
Summary of Findings
The data shows that the 100% concentration of Lemongrass oil produced a larger zone of inhibition on the 4th day of culture of the
Trichophyton rubrum however on the 7th day there was a change, 75% concentration of Lemongrass oil extract had the largest zone of inhibition while the smallest zone of inhibition was still the same which is the 25% concentration
Summary of Findings
The triplicate shows that after four days of incubation, 100% lemongrass oil extract produced a larger zone of inhibition with the mean of 36.67mm, higer than the standard with the mean of 28. 67mm, which means that Trichophyton rubrum is susceptible to this concentration. However, on the seventh day of incubation, the zone of inhibitionbecomes narrower giving only the mean of 11.67mm due to some factors.
Summary of Findings
The zones of inhibition of the triplicate plates for the 4th day produced by the Ketoconazole, the 50%, 75% and 100% concentration of Lemongrass showed a variance beyond 0. Therefore the results are valid but lack reliability. mean while the result of the 25% concentration of Lemongrass oil extract exhibits a variance of 0 hence the result is valid and reliable.
Summary of Findings
The zone of inhibition of the triplicate plates for the 7th day produced by the Ketoconazole, the 50%, 75% and 100% concentration of Lemongrass showed a variance beyond 0. Therefore, the result are valid but lack reliability. Meanwhile the result of the 25% concentration of Lemongrass oil extract exhibits a variance of 0.33 hence the result is valid and adequately reliable.
Conclusion
Based on the findings, the P-value exceeds the 0.05 level of significance which there leads the researchers to accept the null hypothesis that there is no significant difference between the zones of inhibition of the Trichophyton rubrum after the inoculation of the of Lemongrass oil extract. this is due to the fact that 25%, 50%and 75% Lemongrass oil extract can inhibit the growth of Trichophyton rubrum but the produced zone of inhibition does not fall within the range of the standard. From the results of the study. The researchers conclude that 100% Lemongrass oil extract produced a zone of inhibition which is comparable to the effect of the standard and therefore can serve as anti fungal agent against Trichophyton rubrum.
Recommendations
A study on the possiblity of other plant sources that could be used as an alternative for anti fungal treatment against Trichophyton rubrum
Use of Minimum inhibitory concentration (MIC) to plot the regression analysis to establish zone size breakpoints to define the categorical limits for susceptible, intermediate, and resistant for anti microbial agent.
The use of phytochemical screening for the quantitative and qualitative analysis of Metabolites of the Lemongrass.
Other uses of Lemongrass oil in the field of Medical Technology such as in immunology as an analgesic that can help reduce pain and inflammation, and also on various Pharmacological activities such as an anti amoebic, anti diarrheal, anti filarial properties.
A study on different methods of prolonging the shelf life of the Lemongrass oil extracts.
Lastly, to formulate a new topical cream using the Lemongrass oil extract as the primary anti- fungal agent.
THANK YOU FOR LISTENING!
Review of Literature
FUNGI
TRICHOPHYTON RUBRUM
LEMONGRASS
KIRBY-BAUER TEST METHOD
Table. 4 Summary of analysis of Variance: Single Factor on the 4th day of culture
Table 5. Analysis of Variance between groups and within groups
Table 6. Tukey HSD Post-Hoc Text of the 4th Day
Table. 6 Summary of Analysis of Variance: Single Factor on the 7th Day of culture
Table 8. Tukey HSD Post-Hoc Test of the 7th Day
(Johri, 2005)
(Osborne et al , 2003)
(Ahkila, 2010)
(Brown, 2015)
(J. Hudzick, 2009)
Herbal Medicine
(Godiyal, 2014)
Trichophyton rubrum
Fungal Infections
Ketoconazole
25% Lemongrass oil extract
50% Lemongrass Oil Extract
75% Lemongrass Oil Extract
100% Lemongrass Oil Extract
(Ellis) 2015
Table . Analysis of Variance between groups and within groups
Full transcript