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BlackWorm vs. Nicotine

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by

Rediet Abate

on 5 April 2011

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Transcript of BlackWorm vs. Nicotine

Effects of Nicotine on Black Worms Introduction Question: How would nicotine effect the pulse rate of a black worm?
Hypothesis: The increase level of nicotine in a black worm system would increase the pulse rate.

Prediction: If there is nicotine in the black worm’s system, then the black worms heart rate will increase. Background Information Closed Circulatory System: where blood flows inside blood vessels and does not face direct contact with other organs and tissues

Control of circulatory system background- Room temperature worms with no nicotine. Black worm background:
Since black worms have a closed circulatory system just like humans, it would be easier for us to be able to manipulate and maneuver the worms around when observing them.

Nicotine:
causes blood vessels to constrict, blood pressure rises, increases heart rate, narrows arteries Methods Independent variable: Two different levels of nicotine (0.0125 mM and 0.025 mM)

Dependent variable: heart rate (measured by counting number of pulses)

Control: worms at room temperature with out any concentration of nicotine We used 18 worms (6 for each concentration)
For each worm, we are placed them in the thin tubes in a Petri dish
Then we used a dropper, added the two different concentrations of nicotine to the dish, then let it set for a minute
Observed the pulse rate and recorded it. DATA By: Rudy Abate
Courtney Valerio
Kelsey Moxey
Christine Tran Control 0.0125 mM 0.025 mM
17 16 12
13 18 20
12 17 19
16 24 20
14 20 22
14 22 20

Mean: 14.3 19.5 18.8
SD: 1.861898673 3.082207 3.488075

Graph Table Discussion Comparison
( treatment1 vs. treatment 2) t-stat p-value

0.0125 mM level -3.514567554 0.003954262

.025mM level -2.78780469 0.011819108

Our speculation to whynicotine impacted circulation

• Nicotine mimics the effects of a neurotransmitter that is responsible for pulsation rate.

• Acetylcholine agonist-increases pulsation rate of the dorsal blood vessel

• Causes the body to activate “flight or fight”


Our results support our hypothesis in which that as the concentration of nicotine increased it als increased activity of circulatory system. With this direct relationship between the nicotine level and rate of blood circulation of the black worm, we can now make the assumptions that this might’ve happened because some chemicals diffused rapidly through the worm’s thin skin while some chemicals don't. Thus, our control group which was no concentration of nicotine, wouldn’t affect the rhythmic muscular contraction of the blackworms head, while the two other levels of nicotine would. • Immediate release of stored fats into the blood stream-stick to blood vessel walls

• Leads to internal nicotine vascularization-creation of new blood vessels that supply oxygen and nutrients to the fats and plaques that have accumulated

• The result is, raised blood pressure, increased heart rate, and tightening of the blood vessels References http://www.ableweb.org/volumes/vol-27/07_Bohrer.pdf http://www.ehow.com/how-does_5387181_smoking-effects-circulatory-system.html http://whyquit.com/whyquit/LinksJBlood.html THE END
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