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staining of muscle and bone

Modified Gomori’s Trichrome Stain Rapid Gomori trichrome –myofibrils Mallory’s PTAH Heidenhain’s iron hematoxylin
by

darlene mae padua

on 28 September 2012

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Transcript of staining of muscle and bone

Staining of Muscle and Bone Modified Gomori’s Trichrome Stain - red
Rapid Gomori trichrome –myofibrils – green
Mallory’s PTAH – blue
Heidenhain’s iron hematoxylin – grey black Staining of Muscle Lissamine Fast Red – Tartrazine Method
Staining of Muscle and Bone Schmorl’s Picro TModified Gomori’s Trichrome Stainhionin technique
Staining of Bone
Modified Gomori trichrome stain showing several ragged red fibers Cytochrome c oxidase stain showing Type-1 lightly stained and Type II fibers, darker fibers, and a few fibers with abnormal collections of mitochondria. Note cytochrome c oxidase negative fibers as usually seen in mitochondrial encephalopathy, lactic acidosis and stroke-like episodes (MELAS). Succinate dehydrogenase staining showing a few ragged blue fibers and intense staining in the mitochondria of the blood vessels Electron microscopy showing abnormal collection of mitochondria with paracrystalline inclusions (arrowhead), osmiophilic inclusions (large arrowhead) and mitochondrial vacuoles (small arrowhead). Muscle
Fascicles
Myofiber = muscle fiber
Myofibrils
Myofilaments
Myosin and actin filaments

Muscle-related terms Reagents: Reagents may be purchased commercially or prepared in the laboratory.
a. Modified trichrome (Ryan-Blue formulation)
i . Chromotrope 2R………………….6.0 g
ii. Aniline blue………………………..0.5 g
iii. Phosphotungstic acid…………….0.25 g
iv. Acetic acid (glacial)……………… 3.0 ml
v. Distilled water…………………..100.0 ml

ingredients. Allow the mixture to stand (ripen) for 30 min at room
temperature. Add 100 ml of distilled water and adjust the pH to 2.5
with 1.0 M HCl. Properly prepared stain will be dark purple in color.
The staining solution should be protected from light. Store in a
glass or plastic bottle at room temperature. The shelf life is at least 24 months.

b. Acid-alcohol
i. 90% ethyl alcohol………………995.5 ml
ii. Acetic acid (glacial)……………….4.5 ml
Prepare by combining the two solutions


Modified Gomori’s Trichrome Stain
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