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Lab meeting 13/6/13

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by

Cary Zhang

on 29 June 2013

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Transcript of Lab meeting 13/6/13

More results
Net effect =
OXTR antagonism
AVP1bR + AVP2R agonism
AVP1aR agonism + antagonism
Results
Carbetocin (OTR agonist): i.c.v, i.p, i.v
Modulation of Anxiety Behaviour in the Elevated Plus Maze using Peptidic Oxytocin and Vasopressin receptor Ligands in the Rat
Plato Mak, Christina Broussard, Kristina Vacy, Jillian H Broadbear
Timeline
Diazepam
GABA receptor
Atosiban
OXTR
AVP1aR
Desmopressin
AVP1aR
AVP1bR
AVP2R
Saline
Saline receptor :)
Surgery
(only for icv)
Elevated Plus maze
Peptide injections
Introduction
OTR activation is anxiolytic
AVPR activation is anxiogenic
Both modulate stress axis
Has multiple receptors:
AVP1A
AVP1B
AVP2
AVP3
EPM
Established as an effective measure of behavioural anxiety
Oxytocin and Vasopressin have been implicated in mood regulation
i.p.
Carbetocin
OXTR
i.c.v
i.p
i.v
i.v
i.v
i.p
i.v
i.c.v
Desmopressin (AVP1A/BR + AVP2R agonist): i.v
Diazepam (GABA agonist, +ve control): i.p
Atosiban (OXTR + AVP1aR antagonist): i.v
Atosiban + Desmopressin
Atosiban + Carbetocin
Net effect =
OXTR agonism? + antagonism
AVP1aR antagonism
Berenice & Cary presents
A Chua and Zhang presentation
The following presentation may contain awesomeness. Chua and Zhang will not be responsible for any motion sickness caused by this or any affiliated presentation. Please secure your safety harness and remain seated
Discussion
Conclusion
Anxiogenic effects mainly mediated by AVP1aR (previous studies showed AVP1bR antagonism is also anxiolytic)




Can look at biochemical markers (e.g. CRF, ACTH, cort...)

Use L/D box (or similar behavioural paradigms)

Repeated exposure to EPM

Light/Dark Box



Analysis of locomotor activity



Better positive control than Diazepam:
Sedative effects
Behaviour
Results
Drugs
Future studies
Full transcript