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Temperature and Respiration in Mealworms

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David Haas

on 23 April 2013

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Transcript of Temperature and Respiration in Mealworms

Measuring the Effect of Temperature on Mealworm Respiration Marie-Elena Cronin
Kathleen McPhillips
David Haas Experimental Design and Methods Results Room Temperature Cold Room Heat Lamps Methods Temperature Mealworms Heat Regulation Cellular Respiration Background Information and Objectives larval form of the mealworm beetle
4 life stages: egg, larva, beetle, adult
bought in container of oatmeal or bran
also known as tenebrio molitor the process of converting the chemical energy of organic molecules into a form immediately usable by organisms.
C6H12O6 + 6O2(g) → 6 H2O + 6 CO2(g) + energy
usually catalyzed by enzymes
in this experiment, we measured the release of CO2 Mealworms are:
ectotherms - heat derived mainly from an external source
poikilotherms - body temperature varies with the environment - Varying Temperature
- Cold Room
analyzer was placed in a cold room where the temperature was between 8-12°C
stepped out of cold room once analyzing
- Heat Lamps
2 were placed over the analyzer
turned on and off to help maintain temperature
temperature kept in between 37-42°C range Discussion Relevance warmer weathers due to global warming
ectotherms make up the majority of the biomass and about 99% of all species
changes in weather could affect ectotherms and the entire ecosystem What Went Wrong Varying Temperature Heat Lamps
Extended use of heat lamps affected the Quibit Respiration Analyzer
Cold Room
Carbon dioxide readings were extremely high from us breathing in a confined area - Higher temperature leads to higher respiration rate
- More drastic increase in same amount of time than the room temperature study
-Slope of Heated values is 0.7100 ppm/s cold temperature and room temperature were consistent
warm temperature was very hard to maintain
we tried to regulate the temperature by turning the lamp on and off - Relatively constant CO₂ Production
- Increase in respiration could have been attributed to organism agitation
- Slope of 0.1885 ppm/s - Decrease in CO₂ production
- Metabolic rate slowing down in response to the environment
- Slope of -0.4606 http://www.nyworms.com/images/mealworm%20lifecycle.JPG -Followed the initial prep we did for the Animal Respiration Lab
Qubit Respiration System
set up LoggerPro software to measure temperature, CO2 and O2 concentrations for 5 minutes
weighed each worm
tested each worm's respiration rate http://upload.wikimedia.org/wikipedia/commons/3/38/Mealworms_in_plastic_container_of_bran.jpg An increase in temperature will cause an increase in respiration rate Hypothesis Recommendations for Future Testing Instead of heat lamps an incubator might be more accurate and less damaging to the equipment
Instead of using a cold room it could be better to use an ice bath to submerge the test chamber and longer hoses to reach the analyzer The Mealworms mealworms were in a container of bran (50 worms)
we picked the worms randomly
the worms varied a little in mass
5 different worms for each temperature range, so they wouldn't be too agitated Room Temperature Analysis Cold Temperature Analysis Heated Temperature Analysis Our Model ANOVA Test Proved Hypothesis According to our data, our original hypothesis was proved correct
Respiration did increase in response to raised temperature
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