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Cellular Respiration Inquiry Lab

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by

Sophie Arcari

on 3 March 2014

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Transcript of Cellular Respiration Inquiry Lab

Background Information
Living organisms must metabolize compounds derived from food to produce energy.
Cellular respiration produces energy by metabolizing glucose in the presence of oxygen while removing carbon dioxide.
Glucose must be converted into ATP in order to be used.
In eukaryotic cells respiration occurs in mitochondria while in prokaryotic cells aerobic respiration occurs in the membrane
Primary Question
How does the rate of cellular respiration in germinating peas compare to the rate of that in pill bugs?
Procedure
1. Fill a 100 ml graduated cylinder with 50 ml of water, add 25 germinating peas and record the amount of displace water. Drain the water, remove the peas and set them aside.
2. Get two vials and place an absorbent cotton ball and put it in the bottom of the vial.
3. Add 1 ml of 15% KOH to the cotton, then put a layer of nonabsorbent rayon on top of that.
4. fill one with the germinating peas, and one with the ml of displaced water, in this case, 9ml of pill bugs.
5. Insert the non- tapered end of the pyrex graduated pipet into the wide end of a stopper so that the tapered end of the pipet points away from the stopper so the pipet extends just beyond the bottom of the stopper.
6. Insert the stopper firmly into the vial, the seal that has been made between the stopper and the vial should be sufficient to prevent the pipet from moving easily. Place a washer over the pipet tip and guide it down until it rests on the stopper.
7. place a thermometer and the vials in a 20 degree Celsius water bath with the pipet tips resting on the edge of the tray. Allow the repspirometers to equilibriate for 10 minutes.
8. ad one drop of food coloring to the exposed tip of each respirometer. carefully shift the two respirometers until they are fully submerged in the water bath.
9. read the respirometers to the nearest 0.01 ml and take the temperature every 5 minutes ad record. calculate the difference for each result and record.
Results
Graph
Analysis Questions
1) the rate of oxygen decreases because it is being used for cellular respiration we know this because it is a key component in cellular respiration.
2) -Amount of KOH
- Temperature
- number of germinating peas
3) The KOH removed the excess CO2 from the vial so if
we wouldn't have had the KOH then there would have been no oxygen to be consumed.
4) it would increase because they give off heat energy. In boiling water we would have no results becasue of denaturation.
5) The seeds are impacted by photosynthesis because they are plants, but we were measuring oxygen consumption and no oxygen is consumed in photosynthesis. Oxygen is only produced in photosynthesis.
Cellular Respiration Inquiry Lab
Hypothesis
If we test the rate of cell respiration in pill bugs compared to cell respiration in germinating peas we will find that the rate in the pill bugs is significantly higher because cellular respiration is the only way to produce ATP in animal cells while plants such as the peas can use photosynthesis as well.
Materials
KOH
cotton balls and non absorbent rayon
pipets
vials
pill bugs
germinating peas
food coloring
thermometer
washers
water baths
By Sophie Arcari and Madison Miller
KOH is corrosive follow safety precautions

Math:
Difference=(initial volume at time 0) - (volume reading at time X)
Corrected difference= (initial pea/pill bug reading at time 0-pea seed/pill bug at time X) - (Acrylic bead difference)
Conclusion
The hypothesis we formed was supported by this experiment. We predicted that the pill bugs would use more oxygen, thus going through more cellular respiration than the pea. This is because the pill bugsuse more of their energy and require more energy to be made through cellular respiration. Some further questions would be how would bacteria compare to the peas and pill bugs, how would light affect the results, and does temperature impact the process.
control group: respirometer with glass beads
independent variable:
temperature
dependent variable:
02 consumed
Pill Bug
0
5
10
15
20
25
30
germinating peas
0
5
10
15
20
25
30
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