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Banana DNA Extraction

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by

aaliyah jones

on 14 October 2012

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Transcript of Banana DNA Extraction

It is chromosomes that carry someone or something's characteristics in a double helix shape. What is DNA? Purpose: Banana DNA Lab To find Banana DNA by adding materials in a bag and maceratinng it.After the materials have been macerated ethanol will be added to release Banana DNA Experimental Design Zip Lock bag
Water
Paperclip Materials Data & Observations Procedure As a result of the procedure three layers were formed in the test tube ( banana, alcohol, and Banana DNA).The Banana DNA that was formed was a thick white cloudy substance. Analysis By: Aaliyah Jones Banana DNA Extraction http://www.scientificamerican.com/media/inline/find-the-dna-in-a-banana_1.jpg http://www.food.gov.uk/3351/images/document/dna2-web.gif To extract Banana DNA. END RESULT Conclusion Test tube
Detergent
Piece of Banana http://cdn1.iconfinder.com/data/icons/fruits/512/Banana.png http://blogs-images.forbes.com/daviddisalvo/files/2011/11/DNA.jpg Fast reaction
DNA is a cloudy white
3 layers in the test tube
macerated banana is a faint yellow
The purpose of this lab was to extract DNA from a banana. In order to extract DNA you must macerate a thumb sized piece of Banana in a zip lock bag.Then weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue maceratingMeasure out 9.0 mL of water and add to the bag.Then continue macerating.See Ms.Leavitt to get 1mL of detergent soap then continue macerating.Once a thick pace called lysate is created transfer it into a test tube.If there is foam in the test tube carefully remove it.With a squeeze bottle squirt about one inch of ethanol into the lysate gently.Gently twirl the test tube to allow DNA to rise up into the alcohol layer.Use a bent up paper clip to pull out the DNA from the alcohol layer.Through out this lab i obseved that the banana changed color due to the fact other substances were added to it which also changed how thick or thin it was.The extracted DNA was in a solid state which allowed it to be visible. Gather all materials
Macerate a thumb sized piece of Banana
Weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue macerating
Measure out 9.0 mL of water and add to the bag.Then continue macerating
See Ms.Leavitt to get 1mL of detergent soap then continue macerating
Once a thick pace called lysate is created transfer it into a test tube
If there is foam in the test tube carefully remove it
With a squeeze bottle squirt about one inch of ethanol into the lysate gently
Gently twirl the test tube to allow DNA to rise up into the alcohol layer.
Use a bent up paper clip to pull out the DNA from the alcohol layer
Full transcript