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Fundamental Biochemistry

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Wade Givens

on 29 April 2010

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Transcript of Fundamental Biochemistry

Fundamental Biochemistry Central Dogma: Prokaryotes Replication - involved with production of DNA duplicates
Transcription - involved in RNA synthesis
Translation - involved in protein synthesis
Differences between prokaryotes and eukaryotes Developed by Francis Crick in 1958
Framework for the storage and transfer of genetic information between biopolymers in living organisms
DNA, RNA, and Proteins
Accomplished through Replication, Transcription, and Translation Central Dogma: Central Dogma
Replication, Transcription, and Translation
Distinctions Regarding Eukaryotic Systems Topics to be discussed include: Eukaryotes Differences Differences in structure and function are not the only differences!

Also differences in DNA replication schemes What is DNA? Nucleic acid that contains genetic blueprints
Consist of two polymers made of sugars connected by phosphodiester bonds
The 2 polymers are held to together by base pairs of adenine and thymine, and guanine and cytosine, except in RNA where the thymine is replaced by uracil
Twisted into a double helix and then supercoiled Prokaryotic DNA Replication Semi-conservative - One parent strand produces a complimentary daughter strand
Single point of initiation - Occurs when an initiator protein is bound to the DnaA box region (AT-rich areas)
This region is known as melted through and is known as the OriC region
This initiates the formation of 2 replication forks that begin initiation in opposite directions
More complex than prokaryotic replication
Multiple sites of initiation
Only occurs during the S-phase
Replication is linear - NOT circular

http://www3.interscience.wiley.com:8100/legacy/college/boyer/0471661791/animations/replication/replication.swf Eukaryotic DNA Replication DNA Replication Prokaryotic DNA Replication
http://highered.mcgraw-hill.com/olc/dl/120073/micro03.swf Transcription
http://highered.mcgraw-hill.com/olc/dl/120080/bio28.swf http://highered.mcgraw-hill.com/olc/dl/120077/bio30.swf
http://highered.mcgraw-hill.com/olc/dl/120077/micro06.swf Translation Diferences Between Prokaryotic and Eukaryotic Transcription and Translation http://highered.mcgraw-hill.com/olc/dl/120077/bio25.swf
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