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Imaging Cells
Transcript
Questions?
Imaging Cells Practical
What We Learned
The Background
The Results
We saw differences in the expression with inducers.
Binary type response
Inconclusive
Background Information
Lessons
- Defining the quantities you want to analyse can be difficult - e.g. gene expression.
- Analysing images can be hard and time consuming itself - Subtracting background and segmentation.
- Given more time we could do more repeat experiments, analyse more cells, inducers separated.
- E. Coli contained two plasmids
- Expression of each plasmid was regulated by an inducer - IPTG or Arabinose.
- One plasmid contained CFP and the other YFP.
The Question
How does the gene expression of the E-Coli change with differing concentrations of each regulator - IPTG and arabinose?
The Experiment
The Approach
- We incubated the cultures at the various concentrations for 2 hours.
- Collect a sample of E.Coli from each sample to view under an epifluorescence microscope.
The Images
Blue Channel shows CFP
Green Channel shows YFP
by Luki, Jonny and Andreas
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