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Restriction Maps
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Restriction Maps
What are they used for?
- Restriction maps are used for obtaining structural information
- Restriction map information is an important part of many techniques that are used to manipulate DNA
Restriction enzymes- recognize and cut DNA at specific sequences of nucleotides, which are called restriction sites.
What are restriction maps?
Restriction maps are a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints by the use of restriction enzymes.
How is DNA mapped?
Through Digestion is one method
A new molecule is like an uncharted territory and a scientist is like an explorer mapping the new territory . Restriction maps are named for the enzymes used as mapping tools.
First, two portions of the DNA sample are individually digested with different restriction enzymes, and a third portion of the DNA sample is double-digested with both restriction enzymes at the same time.
Next, each digestion sample is separated and the sizes of the DNA fragments are recorded.
Using this information, scientists map out the length of each individual DNA fragment and create the final drawing of the restriction map, which includes the positions of the restriction sites.