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Osmotic effects in erythrocytes
1
OSMOTIC EFFECTS IN ERYTHROCYTES
5. REFERENCES
4. DISCUSSIONS
1. http://docsdrive.com/images/academicjournals/jpt/2012/fig1-2k12-46-51.gif
2. http://www.hutchings-traductions.com/images/rbc.jpg
3. http://www.winkelhage.com/images/Cells.3.jpg
4. http://docsdrive.com/images/academicjournals/jpt/2012/fig1-2k12-46-51.gif
4. DISCUSSIONS
Thank You!!!!
"Diagnostic relevence for theTest"
While doing the dilution series, with the special improved measuring techniques can lead the reliability of measured values and whole results of the experiment.
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Graph showing the regression curve for NaCl + Ceftizoxime dilutions
1. INTRODUCTION
- These above so called intake and exit of solid concentrations will be responsible for the determination of regularity process popularly known as osmolarity.
- Osmoles is the total number of solute particles in a solution.
1. INTRODUCTION
1. Hyperosmotic (concentrated salt solution)
2. Isosmotic (normal salt concentration)
3. Hyposmotic (distilled water)
http://www.winkelhage.com/images/Cells.3.jpg
4. DISCUSSIONS
2. Procedures
- At final stage trasfer of supernatent (~900 uL) into cuvettes.
- The obserbance of the samples at 540 nm was measured.
- Graph for NaCl dilutions do not correspond to the sigmoidal curves.
- The value for the sample number 3 and 7 do not follow the decreasing trend.
- No inermediate values are available to create a smooth sigmoidal model.
3. Results
Graph for NaCl/Urea dilutions
Table 2: Pippeting scheme for NaCl/Urea Dilutions
Graph for NaCl Dilution
3. RESULTS
2. Procedures
Table 1: Pipetting scheme for NaCl dilutions
- The table 3 was prepared with the NaCl dilution series and table 4 was prepared with the mixture of NaCl/Urea.
- The samples were prepared in 1.5 mL tubes.
- Dilution series with 30 uL of provided erythrocytes suspension was prepared.
- Samples were centrifuged for 5 minutes at 300 xg.
BSC Applied Biology
Semester 1
Roshan Acharya
1. INTRODUCTION
Animal cells are generally seperated from their environment by cytoplasmic membrane which is generally
semi-permeable/selectively permeable
Intake of useful substances Exit of waste products
OSMOTIC EFFECTS IN ERYTHROCYTES
Structure
1. Introduction
2. Procedures
3. Results
4. Discussion
5. References
OSMOTIC EFFECTS IN ERYTHROCYTES
1. Introduction
Hypothesis:
"The concentration of solution has effects on erythrocytes tonicity."