Hangátirat Automatikusan generált
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Hi, My name is Caroline.
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My project I've been working on has bean signals of
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selection in Carnival told like presenters, eso toilet protectors are
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the first line of defense in DNA immune system.
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They detect, uh, components from pathogens such as viruses or
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bacteria. The key area within a toll like receptor that's
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responsible for the detection is this loosen rich for Pete
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region. So compared to the rest of the toilet receptor
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is really highly variable on dhe.
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Unlike the rest is not under very strong purifying selection.
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I'm expecting to find the most positively selected code owns
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within the loosen retro Pete region on.
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I'm also going to be looking for differences between toll
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like receptors, depending on whether they are receptive to viral
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components or non vital components.
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I'm also looking for any unique trends or if the
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trend seen in carnivores, uh, match up with those off
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other animals.
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So to carry out my research, I collected as many
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toll like receptor sequences from carnivores as I could.
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Um, I had a total of 51 different species, and
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from these, uh, sequences, I created alignment and then carried
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out five different tests on the alignment.
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The sites that were identified with each test from each
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test were compared, and if a two or more tests
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were in agreement on a site than I considered the
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site as potentially under positive selection.
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So the results were that all T.
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L R's, apart from toll like receptor seven were under
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positive selection in carnivores, E found the toll like receptor
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four had the most positively selected code ons on this
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is also being found in other studies off mammals.
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Old toll like receptors that were under positive selection had
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at least one positively selected code on within the loosen
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rich repeat region.
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But in total, 50 of the 69 politically selected code
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on were found in the losing rich repeat region, which
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matched my prediction.
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Three off the four viral scenting toll like receptors were
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found to have positive selection.
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Eso This is a bit unusual compared to what other
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studies have found, so kind of was a potentially experiencing
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more positive selection from caused by viral components.
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The nonviolent tear Lars did have mawr positively selected code
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owns, on average than the viral ones, which suggests that
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non verity allows more prone to positive selection or have
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more relaxed purifying selection.
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So in the future, I'm aiming to investigate whether the
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positively selected code ons and their corresponding amino acids have
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any impact on the function of the proteins, which can
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hopefully give more context.
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How significant changes at the positively select code on south
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to the diagram to the right eyes comparing two different
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sites in toll like receptor four.
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So site 341 on 342 thes sites are right next
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to each other within the losing retro Pete region.
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But you can see that 3 41 is completely Uniforms
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on 3 42 has a large amount of variation on
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this under positive selection.
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Ah, nde In this diagram here you can see where
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each domain lies within each toll like receptor.
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This is based on the domains of the domestic cat,
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but you can see that across this selection of From
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here, the majority of the sites are found within the
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loosen rich repeat region on.
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You can also see just how numerous they are until,
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like respectable.
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So thank you so much for looking at my poster.
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If you have any questions or suggestions or improvements, my
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emails just up the top, thank you