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Sequence of Events
Pluronic F127 instead of BSA
Chemistry is not just mix-mix-swirl-swirl
Some... Technical Details
Learn the synthesis procedure
Nanoparticles CAN be synthesised with small radii
Learning Outcomes
Spectral Analysis - Trend and Calibration Curves
Successful experiment: the cumulation of engineering foundations
Time management, planning, strategising
Success or Not?
September '13
December '13
Caculation of amounts to react: 5 minutes
... and WHICH papers to review
The need for literature reviews
Preparation of reagents for desolvation: 30 minutes
The objective of the project is to...
???
Desolvation: 20 minutes per sample
But first, laboratory safety and synthesis procedure
Cleaning up + storing samples: 20 minutes
Spectral Analysis: 5 minutes per sample per analyser
Plotting of Curves + Data Analysis: 30 minutes+++
Total for 5 samples: 235 minutes = 4 hours
Further minimise the nanoparticle size
... How?
Experiment Design: What most affects particle size?
Is it feasible? How can I justify?
Questions?
Will it... work?
Many difficulties in carrying out the experiment
Finally, things are working out!
Time is short, not able to carry out all experiments
Haywire results because of...
Particle Size Analyzer shows smaller sized NPs. Success!
The Stock Solutions: A Story of Massive, Massive Error
Assumptions undermined, recheck again and again
Back to the Drawing Board
Jan '14
April '14
Literature Review: What other researchers did
Error minimisation in ALL experiments
Recalculated, remade, rechecked, reconfirmed
Problem: Lab does not have equipment. So, improvise
Research: Learning from unknowns, finding answers to unknowns, encountering new unknowns
Delays, more improvisation, more errors
Time is never on my side
The mindset to independently carry out experiments, to learn from failure, and to be creative and innovative
Success in learning something new