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HMGCS1 Western blot
1
Week of Sept. 3, 2013 lab update
Next week:
Adenovirus experiment
Budget
Still need to work on miR-455 paper
Kasey had mentioned a qPCR experiment for pri-miR-223
Give you an idea of monthly spending - we need to work together to save as much money as possible
FYI: I leave next Thursday afternoon. I'll be back on Tuesday (really only missing Friday and Monday).
SREBP-1 dominant- negative virus
So this week was expanding up the virus.
Next week: Infect Huh7 (if ready) with virus then treat with LNA-455
HMGCS1 Western blot
Previously worked on HMGCS1 WB but saw no change between 223 KO mice and controls
Kasey gave me some new mouse livers that I homogenized last week.
- did a BCA, diluted samples, then re-did a BCA to check my dilutions - I adjusted sample addition volumes from there